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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 适应物种:
Human, Mouse, Rabbit, Monkey, Pig, Horse, Guinea Pig, Dog
- 库存:
100
- 宿主:
Rabbit
- 应用范围:
IHC-P
- 靶点:
Human NPY1R. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except NPY6R (61%).
- 规格:
50 μg
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文献和实验Construction of a Human Antibody Domain (VH) Library
), called a “domain antibody”, or “dAb”, is approximately four times smaller than a Fab and half the size of a scFv. Here, we describe the construction of a phage-displayed VH library and an approach to introduce genetic diversity in this library, where both diverse
Improvement of Single Domain Antibody Stability by Disulfide Bond Introduction
antibody repertoire. By introducing this particular disulfide bond within a single domain antibody, the conformational stability can be increased in general. In this chapter it is described how to introduce this extra intra-domain disulfide bond
Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies
. We describe here the new generation protocol for intracellular antibody capture to facilitate selection of functional single domains. This protocol uses a series of optimized single domain libraries, based on designed intracellular variable (VH or VL) region scaffolds
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