- $395
- Leading Biology
- AMM01686G
- 2025年09月23日
- WB, IHC, IF, FC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Mouse / IgG1, kappa
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human
- 库存：
100
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF, FC
- 规格：
50 ug
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文献和实验RUVKUN Antibody Staining Protocol revised 10/29/90
above (total volume= 5ml). Then add heptane(or water) and MeOH; EGTA. 100mM Tris Cl pH 7.4 1% Triton X-100 1 mM EDTA 1M H3BO3 0.5 M NaOH 0.1% BSA 1X PBS 0.5% Triton X-100 0.05% Na Azide 1mM EDTA
Staining protocols for placental explant cultures
there is less likelihood of damaging the culture during processing. Incubate overnight (at least) at 4°C in blocking solution (4% BSA/PBS). Include 0.02% sodium azide if the plates are to be kept longer. Incubate in 1st antibody for 30 min at room temperature. Ideally
Staining protocols for placental explant cultures
over the explant, preventing it from drying out. Antibody should be diluted in 4% BSA/PBS. A good positive control antibody is anti-cytokeratin 7 (OV-TL 12/30; Dako; use at 1/50). This identifies the outgrowing cells unequivocally as trophoblast. Anticytokeratin
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