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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 库存:
100
- 宿主:
Rabbit
- 靶点:
Human GRM3 / MGLUR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 抗体名:
GPRC1C, MGLUR3
- 规格:
50 μg
99kDa
细胞定位:
Antigen Cellular Localization: Cell membrane; Multi-pass membrane protein
形式:
Liquid
参考文献:
Makoff A.,et al.Brain Res. Mol. Brain Res. 40:55-63(1996). Sartorius L.J.,et al.J. Neurochem. 96:1139-1148(2006). Hillier L.W.,et al.Nature 424:157-164(2003).
产品概述:
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GRM3 / MGLUR3 antibody (N-Terminus).
功能:
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.
总结:
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.
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文献和实验标本。 2. 加20ul 1 N HC I ,盖紧,上下混匀。 2 - 8 ℃ 放置60± 2 分钟。 3. 加20ul 1 N NaOH, 盖紧,上下混匀。 4. 即用,或放-20/-70 ℃ 保存3 天。计算结果时乘 以稀释倍数 50 。 ( 注意:不同的标本E2F1 的水平可能有较大差异,请根据实际情况灵活掌握稀释度) 5. 细胞培养上清或组织匀浆 10 倍稀释 (410ul 的标本
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
, 50 ml + NaOH, 1N, 100 µl-> heat to 37ѓC, add ddH2 O up to 250 ml 上一篇:Antibody Conjugation Protocol 下一篇:Expanding Hybridoma Clones
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