优秀代理商 凯杰 QIAGEN 217004 miRNe

凯杰 qiagen 217004 miRNeasy Mini Kit (50)  



从多种动物组织和细胞中纯化microRNA和总RNA

For 50 preps: 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers

  

• 有效纯化miRNA和总RNA
• 高效富集miRNA和< 200个核苷酸的RNA
• 高纯度RNA适合多种下游应用
• 灵活的操作方法可用于共纯化或单独纯化各组分

miRNeasy Mini Kit可从多种类型的动物组织和细胞，包括难裂解的组织中纯化18个核苷酸（nt）以上的总RNA，也可单独纯化富集后的miRNA组分和总 RNA（>200 nt）组分（单独纯化需要RNeasy MinElute Cleanup Kit）。miRNeasy Mini Kit利用离心柱进行低通量RNA纯化。miRNeasy Mini Kit可在QIAcube全自动核酸纯化议上自动完成miRNA与总RNA的共纯化。 


 

Product Details

miRNeasy Kits enable purification of total RNA from all types of animal tissues and cells, including difficult-to-lyse tissues and small tissue and cell samples. Alternatively, an miRNA-enriched fraction and a total RNA (>200 nt) fraction can be purified separately (for separate purification, an RNeasy MinElute Cleanup Kit is required when using the miRNeasy Micro or Mini Kit and an additional RNeasy 96 plate when using the miRNeasy 96 Kit). Purification of 1–12 samples can be automated on the QIAcube Connect. High-throughput processing in convenient 96-well format can be performed using centrifugation or vacuum procedures.

The NEW miRNeasy Tissue/Cells Advanced Mini Kit – get excellent results faster without phenol/chloroform using our QIAzol free Advanced chemistry. Try it today.

Principle

miRNeasy Kits combine phenol/guanidine-based lysis of samples with silica membrane-based purification of total RNA. QIAzol Lysis Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction.

miRNeasy Micro and Mini Kits are automatable on the QIAcube Connect. The miRNeasy 96 Kit enables vacuum and/or spin processing.

 

Procedure

miRNeasy Kits enable purification of total RNA, which includes RNA from approximately 18 nucleotides (nt) upwards. Alternatively, an miRNA-enriched fraction and a total RNA (>200 nt) fraction can be purified separately (see flowchart " miRNeasy procedures"). Total RNA, including miRNA, is highly suitable for use in miRNA quantification by real-time RT-PCR. Enrichment of small RNAs in a separate fraction may be advantageous for certain applications where mRNA and rRNA could lead to increased background. This specialized protocol requires use of the RNeasy MinElute Cleanup Kit with the miRNeasy Micro or Mini Kit. If using the miRNeasy 96 Kit, 2 RNeasy 96 plates are required for every 96 samples.

Automated miRNA purification on the QIAcube Connect

Purification of total RNA, including miRNA, from the aqueous phase after phase separation can be fully automated on the QIAcube Connect using the miRNeasy Micro or Mini Kit (see figure " High, reproducible recovery of miRNA from small cell numbers"). The innovative QIAcube Connect uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into your laboratory workflow. Sample preparation using the QIAcube Connect follows the same steps as the manual procedure (i.e., lyse, bind, wash, and elute), enabling you to continue using the miRNeasy Micro or Mini Kit for purification of high-quality RNA.

 

Applications

miRNeasy Kits allow purification of miRNA with total RNA for use in a variety of applications, including:

• RNA-seq
• Quantitative, real-time RT-PCR (e.g., using the miScript PCR System)
• Northern blot analysis
• Microarray analysis