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Lifeline^® Iliac Artery Endothelial cells, when grown in Lifeline^® VascuLife ^® culture medium, provide a low-serum culture ideal for the study of atherosclerosis and other cardiovascular diseases, general vascular biology research, wound healing and cell migration studies.

• Human Iliac Artery Endothelial Cells are isolated in a specialized endothelial cell medium called VascuLife® EnGS Medium to ensure that they remain unexposed to recombinant human VEGF. This specialized medium provides a low-serum (2%) environment with or without VEGF that results in optimal endothelial cell growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium.

Lifeline^® iliac artery endothelial cells are plated and expanded in culture vessels twice before being harvested for cryopreservation to ensure the highest viability, purity, and plating efficiency.

• These cells are not exposed to antimicrobials or phenol red when cultured in VascuLife® medium.

Iliac atery endothelial cells are quality tested for:

• Proliferation and Morphology:  Normal cell appearance for 15 population doublings
• Cell Viability:  Minimum 70% viability when thawed from cryopreservation
• Sterility Testing:  Negative for mycoplasma, negative for bacterial and fungal growth
• Virus Testing:  Negative for HIV-1, HIV-2, HBV and HCV by PCR
• Specific Staining:  von Willebrand factor positive. Smooth muscle α-actin negative.

500,000 cells per vial