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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Normal Human Pulmonary Artery Smooth Muscle Cells
- 细胞类型:
人正常原代细胞
- 物种来源:
人源
- 器官来源:
人源
- 运输方式:
干冰或液氮
- 年限:
液氮储存10年以上
- 生长状态:
冻存
- 规格:
500,000 cells/vial
Lifeline® normal Human Pulmonary Artery Smooth Muscle Cells (HPASMC), when grown in VascuLife® SMC Medium, provide an ideal low serum (5%) culture model for the study of angiogenesis, atherosclerosis, diabetes or vascular/pulmonary biology.
Lifeline® Pulmonary Artery Smooth Muscle Cells have been isolated from human pulmonary artery, plated and expanded in culture vessels twice before being harvested for cryopreservation to ensure the highest viability, purity and plating efficiency.
Pulmonary artery smooth muscle cells, 100X
Our Human Smooth Muscle Cells are quality tested in VascuLife® SMC Medium and demonstrate optimal low serum growth over a period of at least 15 population doublings at rates equal to or greater than other serum-supplemented media.
Lifeline® HSMC are not exposed to antimicrobials or phenol red when cultured in VascuLife® SMC Medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline® offers these traditional supplements; however, they are not needed, or recommended, to achieve optimal cell performance in most research applications.
- Pulmonary artery smooth muscle cells can be grown without phenol red or antimicrobials when cultured in VascuLife® SMC Medium.
500,000 cells per vial
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文献和实验1、传代前24h先将组织块去除:等到细胞覆盖瓶底80%以上后进行组织块去除操作。可用弯头玻璃吸管将组织块轻轻挑起吸出,确保无组织块留下,以防坏死污染。2、胰酶消化:倒掉旧培养液,用D-HANKs液清洗两边,加入已预热(37度)10min的0.25%胰酶消化液2ml,2-3min后镜下观察,发现胞质收缩,细胞间隙增大,立即用含胎牛血清的培养液终止消化。3、弯头吸管吹打细胞:吹打一定时间后到镜下观察,在没吹打起细胞区域继续吹打,动作要柔和。4、离心:2000转5min。倒掉上清液,加入培养液,吹
亦称肺动脉干。在呼吸空气的脊椎动物中,把静脉血由心脏导向肺脏的动脉。在两栖类,1对第四大动脉弓(与第四鳃弓相应的)不与背大动脉汇合而入肺,形成肺动脉。在爬行类。一般也起源于第四大动脉弓,由心室发出时是1条肺动脉干,与左右大动脉弓共同构成动脉干,然后分成左右肺动脉。鳄类、鸟类以及哺乳类随着左右心室的分化,肺动脉由右心室发出。
一、摘要 目的:建立兔膀胱平滑肌细胞的分离、培养和鉴定的方法。方法:成年新西兰白兔两只(正常及梗阻各一只),采用酶法分离技术获得膀胱平滑肌细胞后于10%小牛血清的DMEM中培养,观察细胞形态和扩增情况,用爬片染色、电镜、蛋白质α-肌动蛋白(α-actin)鉴定细胞类型。结果:倒置显微镜下观察均呈“谷和峰”样结构、细胞爬片HE染色及电镜检查均证实为平滑肌细胞。免疫组化染色检测α-actin呈阳性反应。从细胞爬片HE染色和免疫组化染色检测α-actin呈阳性反应中我们发现该方法所的膀胱平滑肌细胞
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