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文献和实验ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
You will need: 13.5 day pregnant mouse (we use MTK NEO inbred white mice) 2 sets sterile instruments one containing a pair of curved forceps and a pair of iris scissors one containing two pairs curved forceps, one pair iris
Euthanasia Euthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress
1-cell embryo transfer into pseudopregnant recipient female mouse
/ bursa before tearing the hole to reduce any bleeding that may occur. Once there is a hole of sufficient size to reach the infundibulum, grasp it at the end with one pair of pattern 55 forceps while inserting one blade of the other set into the tube
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