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文献和实验Dynabeads® Co-Immunoprecipitation Kit
, although the coupling reaction also works well with antibodies in storage buffers that include protein additives (e.g. BSA) and/or sodium azide (NaN3). This kit is not recommended for use with antibodies that have been stabilized in glycerol. 实验试剂
实验原理 The isolation protocol relies on base pairing between the polyA residues at the 3’ end of most mRNA, and the oligo (dT)25 residues covalently coupled to the surface of the Dynabeads® . Other RNA species lacking a polyA
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
strain of E.coli be used for routine plasmid isolation. Examples of such strains include DH5"® and JM109®. 2. Pellet 1.5-5 ml bacteria in appropriate vessels by centrifugation at 10,000 × g for 1 min at room temperature. 3. Decant or aspirate
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