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文献和实验Protocol for Trichloroacetic Acid (TCA) Precipitation
Introduction The efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replication) can be determined by trichloroacetic acid (TCA) precipitation. TCA
Phosphoamino acid analysis:Mark Kamps method
Phosphoamino acid analysis:Mark Kamps's method 1. Label your protein with 32Pi. Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to Immobilon-P .
Preparation of nucleic acid probes
Preparation of nucleic acid probes In standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as single-stranded or double-stranded molecules (see Figure 5.1 ), but the working probe
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