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文献和实验Purification of Endotoxin Free mAbs
from ascitic fluid preparations). To make a 10x stock of buffer A: Malonic Acid 104g NaOH 60g Betaine 20g Make to 2 litres with endotoxin free water
international. Specific activity of the conjugate ranges from 4 to 7 Cryptates per antibody. This conjugate is supplied in 50 mM hepes buff er pH 7.0, 0.1% bovine serum albumin (BSA) protease free. The concentration of this conjugate has been calibrated
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
-galactopyranoside (X-gal; 25 mg/mL in DMF [dimethylformamide]) Agarose gel (1%, UltraPure; Invitrogen) Antibody, specific for the protein of interest Bead eluting buffer Bovine serum albumin (BSA; 0.5% in PBS; New England Biolabs) Cell
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