重组牛肠激酶轻链(带His标签)北京品牌

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产品名称：重组牛肠激酶轻链(带His标签)
英文名称：Recombinant Bovine Enterokinase Light Chain, His
产品货号：GS4881
产品规格：100IU

分子量：Approximately 28kDa,a single non-glycosylated polypeptide chain containing 241 amino acids，with 6×His at C-terminus.
储存条件：冷冻（-20℃）

概述

Enterokinase(EK)is an amino protease existing in duodenum of mammal and is involved in digestion.It consists of a disulfide-linked 82-140 k Da heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35-62 k Da light chain which contains the catalytic subunit.Additionally,both of the chains are derived from a single precursor that is cleaved by a trypsin-like protease.EK can specially recognize the amino acid sequence DDDDK,and digest the peptide bond after the lysine residue.rEK was report to be more effective than nature EK in cleaving recombinant proteins.Furthermore,the light chain possesses the whole enzyme activity of EK.rBoEK has the highest activity than EK of other species and is used wildly in biochemical applications.rBoEK with 6×His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Sterile liquid.
来源	Escherichia coli.
成分	50mMTris-HCl,pH 8.0,0.5MNaCl and 50%glycerol.
内毒素	Less than 1 EU/μg of rBoEKL,His as determined by LAL method.
Unit Definition	One unit is defined as the amount of enzyme needed to cleave 50μgof fusion protein in 16hoursto 95%completion at 25 ℃ in a buffer containing 25 mM Tris-HCl,pH 7.6,50mMNaCl,and 2 mM CaCl2.

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