相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
23
- 英文名:
Aldolase assay kit
- 保质期:
详见说明书
- 供应商:
上海一研
- 保存条件:
低温
- 规格:
50管/24样
中文名称:醛缩酶测定试剂盒(比色法)
英文名称:Aldolase assay kit
产品规格:50管/24样
发货周期:1~3天
检测指标:醛缩酶;ALD
本试剂盒可测各种动物血清(浆)、组织等样本中醛缩酶活性。醛缩酶(ALD)广泛存在于各种组织中,所以当这些组织有病时在血清中会增高,主要用于研究肌肉和肝脏疾病。此酶具有很高研究价值,进行性肌营养不良症可明显增高,常常可达正常值的5~10倍。在急性肌肉坏死时, 此酶也明显增高。但由于神经原因引起的肌肉萎缩时则醛缩酶活力正常。
优点如下:
1、快速简便:全程约1小时,可测50例左右样本。
2、稳定性好:试剂盒2~8℃存放6个月有效。
3、再现性好:变异系数CV=1.2%。
4、回收试验: X =104%。
5、受外界影响因素小:干扰因素少,重复性强。
醛缩酶测定试剂盒(比色法) 细胞生物学研究有以下几个方面:
细胞生物学的研究内容十分广泛,主要包括:
①细胞核、染色体以及基因表达的研究;
②生物膜与细胞器的研究;
③细胞骨架体系的研究;
④细胞增殖及其调控;
⑤细胞分化及其调控;
⑥细胞的衰老与编程性死亡(凋亡);
⑦细胞的起源与进化;
⑧细胞工程.
细胞生物学研究的常用技术有哪些:
1.显微技术:包括显微观察,显微操作.
2细胞组分分析技术:离心分离技术,生物大分子定位技术,定量细胞化学分析技术.
3.细胞工程:细胞培养。
注意事项:
1. 本试剂盒的检测试剂中含有萤光素酶,反复冻融会导致其逐渐失活。尽管经测试本试剂反复冻融5次对于其检测效果无显著影响,为取得良好的使用效果,*次解冻后可适当分装保存。反复冻融过程中,可能会导致检测试剂中出现少量沉淀,此时宜平衡至室温,并尽量溶解。如仍有残留的不溶物,可以离心去除后使用,经测试不会影响后续的检测效果。
2. 检测时需使用白色或黑色的96孔板或384孔板。如果使用普通透明的96孔板或384孔板,相邻孔之间会产生相互干扰。
3. 使用说明中提供了检测ATP标准品的方法,实际检测细胞活力时通常并不需要检测ATP标准品。
Duck Tambusu Virus(TMUV)鸭坦布苏病毒RT-PCR试剂盒别 名 LURAP1L; bA3L8.2; FLJ38505; C9orf150; Chromosome 9 open reading frame 150; CI150_HUMAN; FLJ90271; HYST0841; MGC46502; Uncharacterized protein C9orf150.免 疫 原 KLH conjugated synthetic peptide derived from human C9orf150产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Duck Tambusu Virus(TMUV)鸭坦布苏病毒染料法荧光定量RT-PCR试剂盒别 名 C6orf174; CF174_HUMAN; Uncharacterized protein C6orf174.免 疫 原 KLH conjugated synthetic peptide derived from human C6orf174产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Duck Tambusu Virus(TMUV)鸭坦布苏病毒探针法荧光定量RT-PCR试剂盒别 名 Uncharacterized protein C6orf150; MGC142168; Chromosome 6 open reading frame 150; Hypothetical protein LOC115004; CGAS_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human C6orf150产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
E.coli大肠杆菌通用PCR试剂盒(含致病性和非致病性)别 名 UPF0428 protein CXorf56; CX056_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human CXorf56产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
E.coli大肠杆菌通用染料法荧光定量PCR试剂盒(含致病性和非致病性)别 名 Chromosome 9 open reading frame 152; Hypothetical protein LOC401546; Uncharacterized protein C9orf152; CI152_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human C9orf152产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
E.coli大肠杆菌通用探针法荧光定量PCR试剂盒(含致病性和非致病性)别 名 CRIPT; CRIPT_HUMAN; Cysteine rich interactor of PDZ3; Cysteine rich PDZ binding protein; Cysteine-rich interactor of PDZ three; Cysteine-rich interactor of PDZ3; Cysteine-rich PDZ-binding protein; HSPC139; HSPC139 protein; Hypothetical protein CRIPT; Postsynaptic protein CRIPT.免 疫 原 KLH conjugated synthetic peptide derived from human CRIPT产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Eastern Equine Encephalomyelitis Virus(EEEV )东部马脑脊髓炎病毒RT-PCR试剂盒别 名 CADH9_HUMAN; Cadherin 9 type 2; Cadherin-9; Cadherin9; CDH 9; CDH9; T1 cadherin.免 疫 原 KLH conjugated synthetic peptide derived from human Cadherin 9产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Eastern Equine Encephalomyelitis Virus(EEEV )东部马脑脊髓炎病毒染料法荧光定量RT-PCR试剂盒别 名 Chromosome X open reading frame 21; FLJ11577; Hypothetical protein LOC80231; Uncharacterized protein CXorf21; CX021_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human CXorf21产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Eastern Equine Encephalomyelitis Virus(EEEV )东部马脑脊髓炎病毒探针法荧光定量RT-PCR试剂盒别 名 bA507D14.1; Chromosome 9 open reading frame 153; Hypothetical protein LOC389766; MGC131702; Uncharacterized protein C9orf153;免 疫 原 KLH conjugated synthetic peptide derived from human C9orf153产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Ebola Virus(EBOV)埃博拉病毒RT-PCR试剂盒别 名 C4orf19; CD019_HUMAN; Chromosome 4 open reading frame 19; FLJ11017; LOC55286; Uncharacterized protein C4orf19.免 疫 原 KLH conjugated synthetic peptide derived from human C4orf19产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Ebola Virus(EBOV)埃博拉病毒染料法荧光定量RT-PCR试剂盒别 名 chromosome 4 open reading frame 27; FLJ20534; FLJ33423; FLJ42042; UPF0609 protein C4orf27; CD027_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human C4orf27产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Ebola Virus(EBOV)埃博拉病毒探针法荧光定量RT-PCR试剂盒别 名 Chromosome 4 open reading frame 22; Hypothetical protein LOC255119; MGC35043; Uncharacterized protein C4orf22; CD022_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human C4orf22产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Echinococcus granulosus细粒棘球绦虫PCR试剂盒别 名 mAtNOS1; Chromosome 4 open reading frame 14; hAtNOS1; Hypothetical protein LOC84273; MGC3232; Putative ortholog of Arabidopsis mitochondrial nitric oxide synthase; Uncharacterized protein C4orf14; NOA1_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human C4orf14产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Echinococcus granulosus细粒棘球绦虫染料法荧光定量PCR试剂盒别 名 CD111; CD111 antigen; CLPED1; ectodermal dysplasia 4 (Margarita Island type); ED4; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig like receptor; Herpesvirus Ig-like receptor; HIgR; HveC; Nectin 1; Nectin-1; OFC7; OROFACIAL CLEFT 7; Poliovirus receptor related protein 1; poliovirus receptor-like 1; Poliovirus receptor-related protein 1; PRR; PRR1; PVRL 1; PVRL1; PVRL1_HUMAN; PVRR; PVRR1; SK-12.免 疫 原 KLH conjugated synthetic peptide derived from human PVRL1/CD111/Nectin1产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Echinococcus granulosus细粒棘球绦虫探针法荧光定量PCR试剂盒别 名 caspr 1; Caspr; Caspr1; Cntnap 1; Cntnap1; CNTP 1; CNTP1; CNTP1_HUMAN; Contactin associated protein 1; Contactin-associated protein 1; MHDNIV; NCP 1; NCP1; Neurexin 4; Neurexin IV; Neurexin-4; Nrxn 4; Nrxn4; p190; Paranodin.免 疫 原 KLH conjugated synthetic peptide derived from human CASPR/Neurexin4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
TRAP/Tartrate Resistant Acid Phosphatase 端粒酶调节相关蛋白抗体Rhesus antibody Rh规格 0.1ml
Transthyretin 转甲状腺素蛋白抗体Rhesus antibody Rh规格 0.1ml
Transportin 1 转运蛋白1抗体Rhesus antibody Rh规格 0.2ml
Transketolase(NT) 转酮醇酶(转羟乙醛酶)抗体(N端)Rhesus antibody Rh规格 1ml
Transketolase(CT) 转酮醇酶(转羟乙醛酶)抗体(C端)Rhesus antibody Rh规格 1ml
醛缩酶测定试剂盒(比色法) KAT4 细胞周期基因1蛋白抗体 50次Semen torreyae榧子PCR鉴定试剂盒 负20度 一年2-4周 低温
人内肽-2(EM-2)ELISA试剂盒群多普利标准品
CC趋化因子受体1检测试剂盒Demethoxycurcumin进口/分装萘标准品萘标准品48T/96T细胞株1x10^6cells
CD45/B220 白细胞共同抗原抗体进口/国产大鼠基质金属蛋白酶13(MMP-13)ELISA Kit,48T/96T
CD45/B220 白细胞共同抗原抗体进口/原装人基质金属蛋白酶2/明胶酶A(MMP-2)ELISA Kit,48T/96T
胰蛋白酶检测试剂盒中药对照药材进口/原装枸椽哌标准品枸椽哌标准品48T/96T特价供应规格:
胰蛋白酶原Ⅱ检测试剂盒中药对照药材进口/分装酚磺酞标准品酚磺酞标准品48T/96T特价供应规格:
AFP 胎蛋白AFP抗体进口/分装小鼠基质金属蛋白酶2/明胶酶A(MMP-2)ELISA Kit,48T/96T
胰蛋白酶原激活肽检测试剂盒中药对照药材特价促销恩曲他滨标准品恩曲他滨标准品48T/96T特价供应规格:
CD83 CD83抗体特价促销大鼠基质金属蛋白酶2/明胶酶A(MMP-2)ELISA Kit,48T/96T
胰岛素检测试剂盒中药对照药材进口/国产盐那拉曲坦标准品盐那拉曲坦标准品48T/96T特价供应规格:
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验[基本原理] MTT法是Mosmann 1983年报道的,以后此方法得到了迅速发展并广泛应用于临床与科研研究。其原理是活细胞内线粒体脱氢酶能将四氮唑化合物(MTT)由黄色还原为蓝黑色的甲肷,甲肷的生成量与细胞的增殖程度呈正相关。后者溶于有机溶剂(如二甲亚枫或酸化异丙醇等),并可在560nm波长下进行检测。 [试剂及材料] (1)MTT:用PBS配成5mg/ml的存储液,过滤除菌,4℃保存。 (2) 溶剂:二甲
[基本原理] XTT比色法有Scudiero等首次采用,用于检测细胞增殖。XTT是异种与MTT类似的四唑氮衍生物,可被活细胞线粒体脱氢酶还原成水溶性的棕色甲肷产物,当XTT与电子偶合剂共同使用时,甲肷的生成量与细胞的增殖程度呈正相关。 [试剂及材料] (1)XTT:用60℃预热培养液配制成6.6mmol/L,过滤除菌,现配现用。 (2)吩嗪二甲酯硫酸盐(PMS):用PBS配制成220mmol/L,4℃避光保存20天。 (3) XTT/PMS:XTT与PMS
一、目的植物体内的还原糖主要是葡萄糖、果糖和麦芽糖。它们在植物体内的分布,不仅反映植物体内碳水化合物的运转情况,而且也是合成其它成分碳架来源和呼吸作用的基质。此外,水果、蔬菜中含糖量的多少,也是鉴定其品质的重要指标。其它碳水化合物,如淀粉、蔗糖等,经水解也生成还原糖。因此,测定还原糖的方法在研究植物体内生理生化变化和测定植物体内碳水化合物方面都是很重要的。二、原理还原糖是具有羰基(>C=O)的糖,能将其它物质还原而其本身被氧化。(1) 还原糖在碱性条件及有酒石酸钾钠存在下加热,可以定量地还原
技术资料暂无技术资料 索取技术资料









