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GSSG和GSH检测试剂盒

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  • 询价
  • 上海一研
  • EY-23245
  • 进口、国产
  • 2025年07月11日
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      24

    • 英文名

      GSH and GSSG Assay Kit

    • 保质期

      详见说明书

    • 供应商

      上海一研

    • 保存条件

      低温

    • 规格

      100次

    GSSG和GSH检测试剂盒 细胞生物学研究有以下几个方面:
    细胞生物学的研究内容十分广泛,主要包括:
    ①细胞核、染色体以及基因表达的研究;
    ②生物膜与细胞器的研究;
    ③细胞骨架体系的研究;
    ④细胞增殖及其调控;
    ⑤细胞分化及其调控;
    ⑥细胞的衰老与编程性死亡(凋亡);
    ⑦细胞的起源与进化;
    ⑧细胞工程.
    细胞生物学研究的常用技术有哪些:
    1.显微技术:包括显微观察,显微操作.
    2细胞组分分析技术:离心分离技术,生物大分子定位技术,定量细胞化学分析技术.
    3.细胞工程:细胞培养。
    以下是细胞生物学试剂的详细介绍:

    产品细节图片1
     中文名称:GSSG和GSH检测试剂盒  
    英文名称:GSH and GSSG Assay Kit

    产品规格:共100次
    发货周期:1~3天
    本试剂盒是一种简单易行的可以分别检测出GSH(还原型谷胱甘肽)和GSSG(氧化型谷胱甘肽)含量的检测试剂盒。
    通过谷胱甘肽还原酶把GSSG还原成GSH,而GSH可以和生色底物DTNB反应产生黄色的TNB和GSSG。适当配制反应体系,前后两个反应合并起来后,总谷胱甘肽(GSSG+GSH)就相当于一个颜色产生的限速因素,总谷胱甘肽的量就决定了黄色的TNB形成量。从而通过测定A412就可以计算出总谷胱甘肽的量。用适当试剂先清除样品中的GSH,然后利用上述反应原理就可以测定出GSSG的含量。用总谷胱甘肽(GSSG+GSH)的量扣除GSSG的含量,就可以计算出GSH的含量。
    本试剂盒的具体反应原理如下:
    两个反应相合并:
    还原型谷胱甘肽是绝大多数活细胞中巯基的主要来源,对于维护蛋白巯基适当的氧化还原状态有重要作用,并且是动物细胞中关键的抗氧化剂。总谷胱甘肽中通常90-95%为还原型谷胱甘肽。
    本试剂盒可以检测动物组织、血浆、红细胞、和培养细胞或其它适当样品中GSH和GSSG的含量。 本试剂盒提供了蛋白去除试剂M,可以更加准确地测定出含有蛋白的样品中的GSH和GSSG的量。 本试剂盒的检测下限为0.5微摩尔/升。一个试剂盒共可以进行100次检测,可以测定100个样品的总谷胱甘肽或GSSG的含量,或可以测定出50个样品中GSH和GSSG的各自含量。
    试剂盒组分:
    总谷胱甘肽检测缓冲液——————60ml
    谷胱甘肽还原酶—————————150μl
    氧化型谷胱甘肽 (GSSG)——————5mg
    DTNB——————————————4.5mg
    蛋白去除试剂M——————————1g
    NADPH——————————————4mg
    DMSO———————————————1.5ml
    GSH清除辅助液——————————0.8ml
    GSH清除试剂———————————160μl
    储存条件:-20℃,有效期一年。
    产品细节图片2

    注意事项:
    1. 本试剂盒的检测试剂中含有萤光素酶,反复冻融会导致其逐渐失活。尽管经测试本试剂反复冻融5次对于其检测效果无显著影响,为取得良好的使用效果,*次解冻后可适当分装保存。反复冻融过程中,可能会导致检测试剂中出现少量沉淀,此时宜平衡至室温,并尽量溶解。如仍有残留的不溶物,可以离心去除后使用,经测试不会影响后续的检测效果。
    2. 检测时需使用白色或黑色的96孔板或384孔板。如果使用普通透明的96孔板或384孔板,相邻孔之间会产生相互干扰。
    3. 使用说明中提供了检测ATP标准品的方法,实际检测细胞活力时通常并不需要检测ATP标准品。

    产品细节图片3
    Actinomyces naeslundii内氏放线菌PCR试剂盒别    名  Adropin; C9orf165; Energy homeostasis associated protein; Energy homeostasis-associated protein; ENHO; ENHO_HUMAN; PRO830; UNQ470.免 疫 原  KLH conjugated synthetic peptide derived from human Adropin产品应用   ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用   ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    Actinomyces naeslundii内氏放线菌染料法荧光定量PCR试剂盒别    名  AGPD1_HUMAN; Agphd1; Aminoglycoside phosphotransferase domain containing; Aminoglycoside phosphotransferase domain-containing protein 1; C630028N24Rik; OTTMUSP00000025874; RGD1308677.免 疫 原  KLH conjugated synthetic peptide derived from huamn AGPHD1产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    Actinomyces naeslundii内氏放线菌探针法荧光定量PCR试剂盒别    名  A kinase (PRKA) anchor protein 5; A kinase anchor protein 5; A kinase anchor protein 79kDa; A kinase anchoring protein 75/79; A-kinase anchor protein 5; A-kinase anchor protein 79 kDa; AKAP 5; AKAP 75; AKAP 79; AKAP-5; Akap5; AKAP5_HUMAN; AKAP75; AKAP79; cAMP dependent protein kinase regulatory subunit II high affinity binding protein; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein; H21.免 疫 原  KLH conjugated synthetic peptide derived from human AKAP5产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    Actinomyces pyogenes化脓放线菌PCR试剂盒别    名  Arylsulfatase F; ASF; OTTHUMP00000022857; ARSF_HUMAN.免 疫 原  KLH conjugated synthetic peptide derived from human ARSF产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    Actinomyces pyogenes化脓放线菌染料法荧光定量PCR试剂盒别    名  ATX3L_HUMAN; ATXN3L; Machado-Joseph disease protein 1-like; MJDL; Putative ataxin-3-like protein.免 疫 原  KLH conjugated synthetic peptide derived from human ATXN3L产品应用   WB=1:100-500  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用   WB=1:100-500  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    Actinomyces pyogenes化脓放线菌探针法荧光定量PCR试剂盒别    名  5 AMP activated protein kinase subunit gamma 3; AMPK gamma 3 chain; AMPK gamma3; AMPKG3; Protein kinase AMP activated gamma 3 non catalytic subunit; AAKG3_HUMAN.免 疫 原  KLH conjugated synthetic peptide derived from human AMPK gamma 3产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    Actinomyces spp.放线菌属通用PCR试剂盒别    名  AMP activated protein kinase beta 2 non catalytic subunit; AMPK beta 2; AMPK beta 2 chain; PRKAB 2; Protein kinase AMP activated beta 2 non catalytic subunit.免 疫 原  KLH conjugated synthetic peptide derived from human AMPK beta 2产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    Actinomyces spp.放线菌属通用染料法荧光定量PCR试剂盒别    名  Apoptosis related protein 3; Apoptosis related protein APR 3; APR 3; APR3; Chromosome 2 open reading frame 28; HSPC013; p18; PRO240; ARAID_HUMAN.免 疫 原  KLH conjugated synthetic peptide derived from human APR3/C2orf28 (31-100aa)产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:20-100  IF=1:50-200  IEM=1:20-200    IGS=1:20-200    GICA=1:20-200亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:20-100  IF=1:50-200  IEM=1:20-200    IGS=1:20-200    GICA=1:20-200(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈.
    Actinomyces spp.放线菌属通用探针法荧光定量PCR试剂盒别    名  ATG 18; ATG18; Atg18 protein homolog; ATG18A; WD repeat domain phosphoinositide interacting 1; WD repeat domain phosphoinositide interacting protein 1; WD40 repeat protein interacting with phosphoInositides of 49kDa; WIPI 1 alpha; WIPI 1; WIPI 49; WIPI 49 kDa ; WIPI49.免 疫 原  KLH conjugated synthetic peptide derived from mouse WIPI1产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    Adeno-associated Virus(AAV)腺伴随病毒PCR试剂盒别    名  ATG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.免 疫 原  KLH conjugated synthetic peptide derived from human ATG4A产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    Adeno-associated Virus(AAV)腺伴随病毒染料法荧光定量PCR试剂盒别    名  ANGT_HUMAN; Angiotensin-1; Angiotensin I; Ang I; Angiotensin-2; Angiotensin II; Ang II; Ang III; Angiotensin-3; Angiotensin III; Angiotensin3; Angiotensin 3; Angiotensin-4; Angiotensin 1-4; Angiotensin 1-5; Angiotensin 1-7; Angiotensin 1-8; Angiotensin 2-8; Angiotensin 3-8; Angiotensin 1-9; Angiotensin 1-10; Angiotensinogen; Serpin A8; Angiotensin IV; Ang IV; ANHU; Des-Asp[1]-angiotensin II; SERPINA8.免 疫 原  KLH conjugated synthetic peptide derived from human Angiotensin III产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    Adeno-associated Virus(AAV)腺伴随病毒探针法荧光定量PCR试剂盒别    名  A cyclase III; AC3; adenylate cyclase 3; Adenylate cyclase type III; ADCY3_HUMAN; Adenylate cyclase type 3; ATP pyrophosphate-lyase 3; AC-III; Adenylate cyclase, olfactive type; AC3.免 疫 原  KLH conjugated synthetic peptide derived from human ADCY3。产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    Adenovirus(AV)腺病毒A型PCR试剂盒别    名  adenylate cyclase 7; ATP pyrophosphate-lyase; KIAA0037; AC7; FLJ36387; ADCY7_HUMAN; Adenylate cyclase type 7; ATP pyrophosphate-lyase 7; Adenylate cyclase type VII; Adenylyl cyclase 7.免 疫 原  KLH conjugated synthetic peptide derived from human ADCY7产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    Adenovirus(AV)腺病毒A型染料法荧光定量PCR试剂盒别    名  AC4; Adenylate cyclase 4; adenylate cyclase type 4; Adenylate cyclase type IV; Adenylyl cyclase; ATP pyrophosphate lyase 4; ATP pyrophosphate lyase; ADCY4_HUMAN.免 疫 原  KLH conjugated synthetic peptide derived from human ADCY4产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    Adenovirus(AV)腺病毒A型探针法荧光定量PCR试剂盒别    名  PRKAG 1; PRKAG1; 5 -AMP-activated protein kinase gamma-1 subunit; 5' AMP activated protein kinase subunit gamma 1; AMP activated protein kinase noncatalytic gamma 1 subunit; AMPK gamma 1 chain; AMPKg; MGC8666; PRKAG 1; PRKAG1; Protein kinase AMP-activated gamma 1 non-catalytic subunit.亚    型  IgG产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    ZO-1  胞质紧密粘连蛋白1抗体(闭锁小带蛋白1)Rhesus antibody Rh规格 0.1ml

    ZnT-1  锌转运蛋白1抗体Rhesus antibody Rh规格 0.2ml
    ZNRF4  锌指/环指蛋白4抗体Rhesus antibody Rh规格 0.2ml
    ZNRF3  锌指蛋白3/环指蛋白3抗体Rhesus antibody Rh规格 0.2ml
    ZNRF2  锌指/环指蛋白2抗体Rhesus antibody Rh规格 0.2ml
    GSSG和GSH检测试剂盒 CKLFSF2 isoform 1  趋化素样因子超家族成员2亚型1抗体 50次Rhizoma typhonii白附子PCR鉴定试剂盒   20度 一年2-4周 低温

    人生长激素释放因子(GH-RF)ELISA试剂盒 泰诺福韦杂质B标准品
    1,4,5-三磷肌醇检测试剂盒Glycitein进口/原装卡拉汀酒石盐K-构体卡拉汀酒石盐K-构体48T/96T细胞株1x10^6cells
    GAPDH(3E12)-Loading Control  3-磷甘油醛脱酶单克隆抗体(内参抗体)进口/国产兔血管紧张素Ⅱ(ANG-Ⅱ)ELISA Kit,48T/96T
    GAPDH   3-磷甘油醛脱酶抗体(内参抗体)进口/原装人角化细胞内分泌因子(KAF)/双调蛋白(AR)ELISA Kit,48T/96T
    嗜粒细胞趋化蛋白Eotaxin 1检测试剂盒对照品进口/原装1,4-脱水梨糖醇标准品1,4-脱水梨糖醇标准品48T/96TTR-222-C-L-R Anti-cath-H/CH(CathepsinH)
    嗜细胞活化趋化因子3检测试剂盒对照品进口/分装左旋多相关物质A标准品左旋多相关物质A标准品48T/96TTR-333-C Anti-cath-K/CK(CathepsinK)
    beta-Actin (Loading Control)  β-肌动蛋白抗体(内参抗体)进口/分装小鼠角化细胞内分泌因子(KAF)/双调蛋白(AR)ELISA Kit,48T/96T
    嗜性粒细胞趋化蛋白检测试剂盒对照品特价促销二醇单月桂酯I型标准品二醇单月桂酯I型标准品48T/96TTR-222-C-L-R-S anti-cath-L/CL(CathepsinL)
    14-3-3(Alpha/Beta/Gamma/Delta/Epsilon)  14-3-3蛋白抗体特价促销大鼠角化细胞内分泌因子(KAF)/双调蛋白(AR)ELISA Kit,48T/96T
    嗜性粒细胞趋化蛋白检测试剂盒对照品进口/国产壬苯醇醚10标准品壬苯醇醚10标准品48T/96TTR-222-C-LCRL-2505 

     

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    • A Rapid Method for the Quantification of GSH and GSSG in Biological Samples

      (GSSG) are biologically important intracellular thiols, and alterations in the GSH/GSSG ratio are often used to assess exposure of cells to oxidative stress. GSH plays an important role in scavenging free radicals and protects cells against several toxic

    • 还原型谷胱甘肽(GSH)与氧化型谷胱甘肽(GSSG)的测定

      GSHGSSG 参照 Anderson等 (1992)。取0.5 g样品,加入3 mL冰冷的6%的偏磷酸(含1 mmol•L-1 EDTA , pH 2.8),冰浴研磨,匀浆液以20,000 g,4 ℃离心15 min,取上清液来马上测定GSHGSSG的含量或储存在-20 ℃下等待测定。总的GSHGSSG含量测定如下:200 μL提取液加 1.2 mL反应液包含400 μL反应液1(110 mmol•L-1 Na2HPO4•7H20, 40 mmol•L-1 NaH2PO4•H2

    • Cell Reports:复旦大学孙宁 / 李晓波团队发现 β-羟基丁酸对铁死亡的表观遗传学调控

      代谢组检测。结果发现,GSH 合成的三种重要氨基酸底物显著降低,通过 GSH/GSSG 含量测定以及 4-HNE 染色,作者确定了 ALF 小鼠的胰腺中存在铁死亡的发生。接下来作者发现,在肝脏、血浆、及胰腺组织的代谢物变化中,共同降低的代谢物只有一种,即 β-HB。 那么,β-HB 与铁死亡之间是否存在相关呢?作者首先在体外进行了实验,结果显示,β-HB 能够显著抑制胰腺细胞系以及外分泌类器官的铁死亡。作者随后将 β-HB 以渗透泵的方式持续对 ALF 小鼠进行给药,结果发现,β-HB 能够显著

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