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- Thermo Fisher
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- 2025年12月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 供应商：
上海善然生物科技有限公司
- 保存条件：
室温
- 规格：
400 reactions
5' C A G ↓ C T G 3'
3' G T C ↑ G A C 5'
Thermo Scientific FastDigest PvuII restriction enzyme recognizes CAG^CTG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.
Thermo Scientific FastDigest PvuII is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis
Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.
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文献和实验Determining the Direction of Replication Fork Movement
, with the enzyme of choice in various brands of agarose. The enzymes that cut to completion in Beckman LE agarose are AvaI, Bam HI, BglII, EcoRV, NcoI, PstI, PvuII, SacII, SnaBI, SpeI, StuI, XbaI and XhoI. (There are likely to be many others that also cut
Mesoplasma florum:Transposome construction
Transposome DNA construction from plasmid cutting Cut the transposome out of the containing plasmid with PvuII enzyme, which cuts at the correct location at the mosaic end. Cut also with an enzyme which produces shorter
Quantification of mRNA Levels Using Ribonuclease Protection Assay
ends (shown by the gray color). ( C ) To prepare synthetic NT-3 mRNA, the plasmid is cut with PvuII , which cuts twice in the plasmid backbone. The presence of the vector-derived PvuII fragment will not interfere
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