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文献和实验Agar Plates for Selection of Clones in Bacteria
Sodium Chloride 5 g Bact. Agar 15 g pH 7.0 Autoclave and store sterile at room temperature Stock solutions: Store all frozen
Agar Plates for Selection of Clones in Bacteria
5 g Sodium Chloride 5 g Bact. Agar 15 g pH 7.0 Autoclave and store sterile at room temperature Stock solutions: Store all frozen in aliquots at -20
Competent cells, calcium chloride method, E. coli, description
preparation, most of the nucleic acid at this point is RNA). Extract the solution once with 5 ml chloroform:isoamyl solution, centrifuge and save the aqueous layer. Add 0.1 volumes of 3 M Sodium acetate, mix, overlay with 2 volumes of 95% ethanol
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