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- 2025年11月20日
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文献和实验In Situ Hybridization Using Digoxigenin Labeled Probes
as black bands. Weaker signals are brown Solutions: 1) 10X PBS 1.3 M NaCl 0.07 M Na2HPO4 0.03 M NaH2PO4 2) Hybridization Buffer 0.6 M NaCl 50 mM Sodium phosphate buffer, pH 6.8 1X Denhardt's (0.02% BSA / 0.02% Ficoll / 0.02
% Tween 20 in PBS. 2. Put blot into Kapak bag cut to slightly bigger size than blot. 3. Add about 5 to 10 ml Stripping buffer. 4. Remove as much air as possible and seal bag. 5. Immerse into 80°C water bath and incubate for 20 min. 6. Rinse blot
Native Chromatin Preparation and Illumina/Solexa Library Construction
, buffers (including buffer EB), and collection tubes. MNase digestion buffer for ChIP MNase stop buffer for ChIP NaCl NaOAc (sodium acetate) (3 M, pH 5.3) Phenol:chloroform (1:1) Phosphate-buffered saline (PBS) (1X
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