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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Applied Biosystems™ MagMAX™ Pathogen RNA/DNA Kit
- 供应商:
上海辅泽商贸有限公司
- 保存条件:
Store at room temperature: 1 bottle of Lysis⁄Binding Solution Concentrate 1 bottle of Wash Solution 1 Concentrate 2 bottles of Wash Solution 2 Concentrate 1 bottle of Elution Buffer Store at 4 °C: 1 bottle of Nucleic Acid Binding Beads Store at -20 °C: 1 bottle of Lysis ENHANCER 5 tubes of Carrier Nucleic Acid
- 规格:
480 preps
MagMAX™ Pathogen RNA/DNA Kit
货号: 4462359
相关应用: Animal Health| 货号 | 单位规格 | 单价 (CNY) | 数量 | |
|---|---|---|---|---|
| 4462359 | 480 preps | 21,420.00 |
规格
| Sample Type (General): | Bacteria, Blood, Liquid Samples (e.g. Serum), Other Samples, Viral Samples |
|---|---|
| High Throughput Compatibility: | Automated Protocols, High Throughput-Compatible, Not High Throughput-Compatible (Manual) |
| Downstream Application: | PCR, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR) |
| Final Product: | Pathogen DNA⁄RNA, Viral DNA⁄RNA |
| For Use With (Equipment): | KingFisher™ Flex Magnetic Particle Processor, MagMAX™ Express Magnetic Particle Processor, MagMAX™ Express-96 Deep Well Magnetic Particle Processor, MagMAX™ Express-96 Standard Magnetic Particle Processor |
| Format: | 96-well plate |
| Isolation Technology: | Magnetic Bead |
| Number of Reactions: | 480 Preps |
| Product Line: | MagMAX™ |
| Product Size: | 480 preps |
| Purification Time: | 45 min |
| Sample Type (Specific): | Any Swab Type, Bacteria, BioMed Diagnostics InPouch™ TF Culture, Cell-Free Fluids, Ear Notches, Feces, Mosquitoe, Parasite Samples, Plasma, Saliva, Serum, Tissue Homogenates, Viral Sample, Viral Transport Media Samples, Whole Blood |
内容及储存
Store at room temperature:1 bottle of Lysis⁄Binding Solution Concentrate
1 bottle of Wash Solution 1 Concentrate
2 bottles of Wash Solution 2 Concentrate
1 bottle of Elution Buffer
Store at 4 °C:
1 bottle of Nucleic Acid Binding Beads
Store at -20 °C:
1 bottle of Lysis ENHANCER
5 tubes of Carrier Nucleic Acid
描述
The Applied Biosystems® MagMAX™ Pathogen RNA/DNA Kit enables purification of RNA and DNA from viruses and easy-to-lyse bacteria and parasites, using magnetic particle technology in a 96-well format. PCR inhibitors are effectively removed in the process, making the resulting nucleic acid ideal for real-time PCR and RT-PCR applications.Key Features:
• Convenience and flexibility: one kit for RNA and DNA, suitable for a wide range of sample types sample input volumes
• Increased confidence in your results: fewer false-negatives due to effective PCR inhibitor removal
• Improved workflow efficiency: process 96 samples in approximately 45 minutes using the MagMAX™ Express-96 Deep Well Magnetic Particle Processor
The MagMAX™ Pathogen RNA/DNA Kit has been optimized to provide a convenient, flexible solution (Fig. 1). This single kit can accomodate:
Purification of RNA and DNA
• Purification from viruses and easy-to-lyse bacteria and parasites
• Low and high sample input volumes
• A wide range of sample types:
• Low cell content samples (e.g., serum and plasma; nasal, tracheal, and cloacal swabs; ear notches)
• Whole blood
• Semen
• Oral fluid
• Feces
• Biomed Diagnostics InPouch™ TF (Tritrichomonas foetus) culture
The MagMAX™ Pathogen RNA/DNA Kit is Easy to Automate
Purify RNA/DNA from 96 samples in approximately 45 minutes (Fig. 2) using the MagMAX™ Express-96 Deep Well Magnetic Particle Processor. Low and high sample input volumes can be used for most sample types, and multiple sample types can be processed at the same time on the same plate after sample-specific pre-processing, saving time and plastics.
Increase the Accuracy of Your Results
Reduce false-negative results due to the effective PCR inhibitor removal achieved by the MagMAX™ Pathogen RNA/DNA Kit. Magnetic particles bind RNA/DNA more efficiently and are more thoroughly washed than glass fiber filters, resulting in higher yields and purity of RNA/DNA. In addition, no sample cross contamination has been observed due to extensive optimization of the MagMAX™ Express-96 Deep Well Magnetic Particle Processor programs.
For Laboratory Use Including Veterinary and Environmental Uses
图表
Comparison of Processing Times
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文献和实验. Proceed step 2. Tips: 1 volume of Buffer P3 and 2 volume of absolute ethanol can be premixed. Then add 1.5 x volume of this mixture into DNA solution. Purify DNA by HiBindTM MicroEluteTM DNA Column 2) Place a MicroElute HiBind™ DNA
BioBrick Assembly Using the In-Fusion PCR Cloning Kit
Synthetic biologists assemble genetic circuits from standardized biological parts called BioBricks™. BioBrick™ examples include promoters, ribosome binding sites, DNA or RNA-coding sequences, and transcriptional terminators. Standard BioBrick
of 10-15 ug DNA. If the expected yield is larger, divide the sample into the appropriate number of columns. 5) Add 300 ul of Binding Buffer (XP2) into the column. Centrifuge at 10,000 x g for 1 min at room temperature to wash the column. Discard






