细胞名称: BHK-21细胞 种属来源: 仓鼠
组织来源: 正常肾
细胞形态: 成纤维细胞
生长特性: 贴壁生长
培 养 基: MEM培养基,90%;FBS,10%。
生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 1
应 用: 世界动物卫生组织(OIE)使用这些细胞对狂犬病的常规诊断。
该BHK-21细胞系已被用来作为一个含有可选择和可扩增标记DNAs表达载体转化的宿主。
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