细胞名称: ATCC HTB-20(BT-474)人乳腺导管癌细胞 种属来源: 人
组织来源: 乳房
疾病特征: 乳腺导管癌
细胞形态: 上皮细胞样
生长特性: 贴壁生长
培 养 基: RPMI-1640,90%;FBS,10%。
生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 1
STR: Amelogenin: X
CSF1PO: 10,11
D13S317: 11
D16S539: 9,11
D5S818: 11,13
D7S820: 9,12
THO1: 7
TPOX: 8
vWA: 15,16
同工酶: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1
PGM3, 1
参考文献: Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572
Lasfargues EY, et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035
Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764
The cells form adherent patches of epithelial-like cells The patches are compact multilayered colonies that rarely become confluent
Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572
特点和简介 E. Lasfargues 和 W.G. Coutinho从一个实心的乳腺浸润性导管癌中分离到了BT-474细胞株。 在本库通过
支原体检测。 在本库通过STR检测。