细胞名称: ATCC CRL-2389(LTPA)小鼠胰腺腺癌细胞 种属来源: 小鼠
组织来源: 胰腺
疾病特征: 胰腺腺癌
细胞形态: 上皮细胞样
生长特性: 贴壁生长
培 养 基: DMEM培养基,90%;FBS,10%。
生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 1
参考文献: Leiter EH, et al. An epithelial cell line with chronic polyoma infection established from a spontaneous mouse pancreatic adenocarcinoma. Cancer Res. 38: 969-977, 1978. PubMed: 205354
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.