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文献和实验. Transfer supernatant (about 4 ml) to a 12 ml disposable centrifuge tube. Add 4 ml ice-cold absolute ethanol. On mixing, the nucleic acids (2% DNA and 98% RNA) and some residual proteins with immediately precipitate. Spin at 10,000 rpm for 10 min
Genomic DNA prep (Jorgensen Lab [modified slightly by Mike H.]) Make worm growth plates. These use agarose to avoid impurities in most batches of agar, and are enriched to allow greater worm growth. Mix: 5 g Bacto
Yeast Genomic DNA Prep Linda Hoskins/Hahn lab Aug 18, 1997 (modified from Philippsen, 1991) Grow 10ml YPD cultures o/n. Figure out cell density; inoculate 30 ml YPD and grow o/n so that cell density is approximately 2 x 108
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