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文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
实验原理 If using the SQ DNA/RNA/Protein for the first time, please read this booklet to become familiar with the procedure and its various modifications. Samples are first lysed in a specially formulated buffer. The protein
of 10-15 ug DNA. If the expected yield is larger, divide the sample into the appropriate number of columns. 5) Add 300 ul of Binding Buffer (XP2) into the column. Centrifuge at 10,000 x g for 1 min at room temperature to wash the column. Discard
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
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