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文献和实验A method is presented for the extraction of total protein from Arabidopsis thaliana tissue. The protocol was designed for the solubilization of a range of proteins and their efficient and quantitative recovery. It is especially compatible
Dynabeads® Co-Immunoprecipitation Kit
Co-Immunoprecipitation Optimization below) from which the desired protein complex is isolated. The Extraction Buffer is made with 5 × IP supplied in the kit. The 5 × IP is supplied as 5 × concentrate and in sufficient quantity to allow for 40 coimmunoprecipitation
PMSF (1 mM) Protease Inhibitor Cocktail, Sigma (Cat. no. P-2714) Quant-iT™ Protein Quantitation Kit (Cat. no. Q33210) Or Cell Extraction Buffer Formulation 100 mM Tris, pH 7.4 2 mM Na3 VO4 100 mM NaCl
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