Phospho-ATF-2 (Thr71) Antibody

价格询价

品牌Cell Signaling Technology
货号
9221
产地USA
更新日期2026年01月01日

应用范围W, IP, IHC-P, IHC-F, IF-IC, F
宿主Rabbit
适应物种H,M,R,Mk

美国CST中国公司(丁香通商铺)

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详细信息
文献和实验
技术资料

抗体英文名：
Phospho-ATF-2 (Thr71) Antibody
抗原：
synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF2
应用范围：
W, IP, IHC-P, IHC-F, IF-IC, F
宿主：
Rabbit
适应物种：
H,M,R,Mk
级别：
详见MSDS文件
库存：
大量
保质期：
详见说明书
供应商：
CST
是否单克隆：
2
保存条件：
-20°c
规格：
100 ul (10 western blots)/300 ul (30 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	300 ul (30 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP IHC-P IHC-F IF-IC F	H M R Mk	Endogenous	70	Rabbit

Protocols	9221: Flow , IHC / Frozen , IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting
Specificity / Sensitivity	Phospho-ATF-2 (Thr71) Antibody detects endogenous levels of ATF-2 only when phosphorylated at threonine 71. This antibody does not cross-react with phosphorylated c-Jun, CREB or other transcription factors. It recognizes both Thr69/Thr71 dually phosphorylated ATF-2 and Thr71 singly phosphorylated ATF-2 equally well.
Source / Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF2. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting Western blot analysis of extracts from HeLa, C6 and NIH/3T3 cells, untreated or anisomycin-treated, using Phospho-ATF-2 (Thr71) Antibody. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma showing nuclear localization, using Phospho-ATF-2 (Thr71) Antibody . IHC-P (paraffin) Immunohistchemical analysis of paraffin-embedded human colon carcinoma using Phospho-ATF-2 (Thr71) Antibody. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-ATF-2 (Thr71) Antibody. IHC-F (frozen) Immunhistochemical analysis of frozen H1650 xenograft using Phospho-ATF-2 (Thr71) Antibody. Flow Cytometry Flow cytometric analysis of Jurkat cells, untreated (blue) or Anisomycin-treated (green), using Phospho-ATF-2 (Thr71) Antibody compared to a nonspecific negative control antibody (red). IF-IC Confocal immunofluorescent analysis of HeLa cells, either untreated (left) or anisomycin-treated (right), using Phospho-ATF-2 (Thr71) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Background	The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2). Abdel-Hafiz, H.A. et al. (1992) Mol. Endocrinol. 6, 2079-2089. Gupta, S. et al. (1995) Science 267, 389-393. van Dam, H. et al. (1995) EMBO J. 14, 1798-1811. Livingstone, C. et al. (1995) EMBO J. 14, 1785-1797.
Application References	deRuiter, N. D. et al. (2000) Ras-dependent regulation of c-Jun phosphorylation is mediated by the Ral guanine nucleotide exchange factor-Ral pathway. Mol. Cell. Biol. 20, 8480-8488. Applications: Western Blotting Hocevar, B. A. et al. (1999) TGF-beta induces fibronectin synthesis through a c-Jun N-terminal kinase-dependent, Smad4-independent pathway. EMBO J. 18, 1345-1356. Applications: Western Blotting Marinissen, M. J. et al. (2001) Regulation of gene expression by the small GTPase Rho through the ERK6 (p38 gamma) MAP kinase pathway. Genes Dev. 15, 535-553. Applications: Western Blotting Ouwens, D. M. et al. (2002) Growth factors can activate ATF2 via a two-step mechanism: phosphorylation of Thr71 through the Ras-MEK-ERK pathway and of Thr69 through RalGDS-Src-p38. EMBO J. 21, 3782-3793. Applications: Western Blotting Suh, Y. et al. (2000) Differential activation of c-Jun NH2-terminal kinase and p38 mitogen-activated protein kinases by methyl methanesulfonatein the liver and brain of rats: implication for organ-specific carcinogenesis. Cancer Res. 60, 5067-5073. Applications: Western Blotting Zhang, S. and Kaplan, M.H. (2000) The p38 mitogen-activated protein kinase is required for IL-12-induced IFN-gamma expression. J. Immunol. 165, 1374-1380. Applications: Western Blotting Hou, N. et al. (2008) Endocrinology 149, 1654-65. Applications: Western Blotting Ogino, T. et al. (2009) Leuk Res 33, 151-8. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products	9223 ATF-2 Control Cell Extracts 9225 Phospho-ATF-2 (Thr69/71) Antibody 9226 ATF-2 (20F1) Rabbit mAb 9224 ATF-2 Fusion Protein 7003 20X LumiGLO^® Reagent and 20X Peroxide 7720 Prestained Protein Marker, Broad Range (Premixed Format) 7727 Biotinylated Protein Ladder Detection Pack 7074 Anti-rabbit IgG, HRP-linked Antibody 7075 Anti-biotin, HRP-linked Antibody For Research Use Only. Not For Use In Diagnostic Procedures.