- 询价
- Cell Signaling Technology
- 9198
- USA
- 2025年10月01日
- western blot,免疫组化（IHC）,免疫组化（IHC）,免疫荧光（IF）,免疫荧光（IF）,流式细胞（Flow Cyt）,染色质免疫沉淀分析（ChIP）
- 兔
- 人,小鼠,大鼠
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-CREB (Ser133) (87G3) Rabbit mAb
- 抗原：
/
- 应用范围：
western blot,免疫组化（IHC）,免疫组化（IHC）,免疫荧光（IF）,免疫荧光（IF）,流式细胞（Flow Cyt）,染色质免疫沉淀分析（ChIP）
- 宿主：
兔
- 抗原来源：
/
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
人,小鼠,大鼠
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/300 ul (30 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (30 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Neuroscience
Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198
PhosphoSitePlus ® protein, site, and accession data: CREB
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IHC-F IF-F IF-IC F ChIP | H M R | Endogenous | 43 | Rabbit IgG |
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-F=Immunofluorescence (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9198:
- ChIP Agarose , ChIP Magnetic , Flow , IHC / Frozen , IHC / Paraffin , Immunofluorescence , Western Blotting
Specificity / Sensitivity
Phospho-CREB (Ser133) (87G3) Rabbit mAb detects endogenous levels of CREB only when phosphorylated at serine 133. The antibody also detects the phosphorylated form of the CREB-related protein, ATF-1.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB.
Western Blotting
Western blot analysis of extracts from SK-N-MC cells, untreated or forskolin- and FGF-treated, using Phospho-CREB (Ser133) (87G3) Rabbit mAb (upper) or CREB (48H2) Rabbit mAb #9197 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear staining, using Phospho-CREB (Ser133) (87G3) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse lung using Phospho-CREB (Ser133) (87G3) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-CREB (Ser133) (87G3) Rabbit mAb in the presence of control peptide (left) or Phospho-CREB (Ser133) Blocking Peptide #1090 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded SK-N-MC cells, untreated (left) or IBMX- and forskolin-treated (right), showing induced nuclear staining, using Phospho-CREB (Ser133) (87G3) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-CREB (Ser133) (87G3) Rabbit mAb.
IHC-F (frozen)
Immunohistochemical analysis of frozen H1650 xenograft, showing nuclear localization using Phospho-CREB (Ser133)(87G3) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of SK-N-MC cells, untreated (blue) or IBMX- and forskolin-treated (green), using Phospho-CREB (Ser133) (87G3) Rabbit mAb compared to a nonspecific negative control antibody (red).
IF-IC
Confocal microscopic images of SK-N-MC cells showing nuclear stain after 25 minute treatment with Forskolin and IBMX using Phospho-CREB (Ser133) (87G3) Rabbit mAb (left, red) compared to untreated cells (right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-F
Conofocal immunofluorescent images of rat dentate gyrus, either sham-operated (left) or 15 min ischemia followed by 30 min (center) and 4 h (right) reperfusion, labeled with Phospho-CREB (Ser133) (87G3) Rabbit mAb (red), Neurofilament-L (DA2) Mouse mAb #2835 (blue) and Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854.
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells treated with Forskolin #3828 (30 μM) for 1h and either 20 μl of Phospho-CREB (Ser133) (87G3) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Background
CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ , and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).
- Lonze, B.E. et al. (2002) Neuron 34, 371-85.
- Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
- Redmond, L. et al. (2002) Neuron 34, 999-1010.
- Dash, P.K. et al. (1990) Nature 345, 718-21.
- Yin, J.C. et al. (1994) Cell 79, 49-58.
- Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
- Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
- Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
- Tan, Y. et al. (1996) EMBO J 15, 4629-42.
Application References
- Zaru, R. et al. (2007) Nat Immunol 8, 1227-35. Applications: Western Blotting
- Ghosh, R. et al. (2007) Neoplasia 9, 893-9. Applications: IHC-P (paraffin)
- Kumar, A.P. et al. (2007) Clin Cancer Res 13, 2784-94. Applications: IHC-P (paraffin)
- Gaddini, L. et al. (2009) Neurobiol Dis 35, 278-85. Applications: IF-IC (In Cells) Western Blotting
- Chung, Y.W. et al. (2011) J Biol Chem 286, 29681-90. Applications: Western Blotting
- Hashimoto, Y. et al. (2009) PLoS One 4, e5130. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9191 Phospho-CREB (Ser133) Antibody
- 9193 CREB Control Cell Extracts
- 9196 Phospho-CREB (Ser133) (1B6) Mouse mAb
- 9197 CREB (48H2) Rabbit mAb
- 1090 Phospho-CREB (Ser133) Blocking Peptide
- 7071 Phototope® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 9190 PhosphoPlus® CREB (Ser133) Antibody Kit
- 9187 Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 9104 CREB (86B10) Mouse mAb
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8112 SignalStain® Antibody Diluent
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号；因此，我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂，我们则建议使用与二抗来源相同的血清进行封闭。抗体：Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本：石蜡
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