- 询价
- Cell Signaling Technology
- 3081
- USA
- 2025年12月23日
- W
- Rabbit
- R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-C/EBPbeta (Ser105) Antibody (Rat Specific)
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser105 of rat C/EBPbeta
- 应用范围：
W
- 宿主：
Rabbit
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 适应物种：
R
- 供应商：
CST
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | R | Endogenous | 41 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-C/EBPbeta (Ser105) Antibody (Rat Specific) detects endogenous levels of rat C/EBPbeta only when phosphorylated at serine 105. It does not cross-react with phosphorylated rat C/EBP family members alpha, delta, gamma, epsilon or zeta. It also does not recognize the p20 LIP rat C/EBPbeta isoform. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser105 of rat C/EBPbeta. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from untreated or serum stimulated PC12 cells, using Phospho-C/EBPbeta (Ser105) Antibody (Rat Specific) (A, B) or C/EBPbeta Antibody #3082 (C, D). Nitrocellulose membranes B and D were treated with alkaline phosphatase (CIP) to show the phospho-specificity of Phospho-C/EBPbeta (Ser105) Antibody. |
| Background | CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal functions and inflammatory response (1). Six members of the family have been characterized (C/EBPα, -β, -γ, -δ, -ε and -ζ) and are distributed in a variety of tissues (1). There are two forms of C/EBPβ, the 38 kDa liver activating protein (LAP) and the 20 kDa liver inhibitory protein (LIP) which may be products of alternative translation. The 38 kDa LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPβ transcriptional activity (2). Phosphorylation of C/EBPβ at distinct sites stimulates its transcriptional activity (3-5). Phosphorylation at serine 105 of rat C/EBPβ, a unique site only present in the rat sequence, seems essential for rat C/EBPβ activation (6).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Screening of Antigen-Specific Antibody-Secreting Cells
Screening of antigen-specific antibody-producing cells is a key step for obtaining antigen-specific monoclonal antibodies. In murine system, hybridoma between B-lymphocytes and myeloma cells is used to screen and produce antigen-specific
specific antibody can be obtained from each rat. Furthermore, when nude rats are used for ascites production, the levels of endogenous immunoglobulin can be less than 1 mg/mL.
Antibody Targeting of Nanoparticles to Tumor-Specific Receptors: Immunoliposomes
Immunoliposomes generated by coupling of antibodies to the liposomal surface allow for an active tissue targeting, e.g., through binding to tumor cell-specific receptors. Instead of whole antibodies, single-chain Fv fragments (scFv
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