- 询价
- Cell Signaling Technology
- 8843
- USA
- 2025年12月19日
- W
- H,M,R,Mk,X,Hr
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-MOB1 (Thr12) (D2E3) Rabbit mAb
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Thr12 of human MOB1 protein
- 应用范围：
W
- 库存：
大量
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 适应物种：
H,M,R,Mk,X,Hr
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey X=Xenopus Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk (X) (Hr) | Endogenous | 24 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-MOB1 (Thr12) (D2E3) Rabbit mAb recognizes endogenous levels of MOB1 protein only when phosphorylated at Thr12. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr12 of human MOB1 protein. Western Blotting
Western blot analysis of extracts from MCF7 cells, untreated or H2 O2 -treated (2.5 mM, 30 min), using Phospho-MOB1 (Thr12) (D2E3) Rabbit mAb (upper) and MOB1 Antibody #3863 (lower). Western Blotting
Western blot analysis of extracts from Ramos cells, untreated or λ phosphatase-treated, using Phospho-MOB1 (Thr12) (D2E3) Rabbit mAb (upper) and MOB1 Antibody #3863 (lower). |
| Background | MOB1 was first identified in yeast as a protein that binds to Mps with essential roles in the completion of mitosis and the maintenance of ploidy (1). Its Drosophila and mammalian homologs, Mats and MOB1, respectively, are involved in the Hippo signaling tumor suppressor pathway, which plays a critical role in organ size regulation and has been implicated in cancer development (2-5). There are two MOB1 proteins in humans, MOB1α and MOB1β, that are encoded by two different genes but have 96.3% identity (6). Both forms bind to members of the nuclear Dbf2-related (NDR) kinases, such as LATS1 and 2 and NDR1 and 2, thereby stimulating kinase activity (7-9). This binding is promoted by the phosphorylation of MOB1 at several threonine residues by MST1 and/or MST2 (5,10). Phosphorylation at Thr12 by MST1/2 stabilizes MOB1, enhancing its binding and regulation of LATS1 (5). The resultant increase in LATS1 kinase activity promotes inhibitory phosphorylation of the transcriptional co-activators YAP and TAZ (11,12), leading to changes in the expression of genes involved in cell cycle progression (13).
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| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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