- 询价
- Cell Signaling Technology
- 8745
- USA
- 2025年12月18日
- W
- H,M,R,Mk,Hm,C,X,B
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
SNF5 (D9C2) Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues surrounding Gln244 of human SNF5 protein
- 应用范围：
W
- 供应商：
CST
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 库存：
大量
- 适应物种：
H,M,R,Mk,Hm,C,X,B
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken X=Xenopus B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk (Hm) (C) (X) (B) | Endogenous | 44 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | SNF5 (D9C2) Rabbit mAb recognizes endogenous levels of total SNF5 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln244 of human SNF5 protein. Western Blotting
Western blot analysis of extracts from various cell lines using SNF5 (D9C2) Rabbit mAb. |
| Background | ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors such as nuclear receptors, p53, Rb, BRCA1, and MyoD facilitate recruitment of the complex to target genes to regulate gene activation, cell growth, cell cycle, and differentiation processes (1,6-9). SNF5, one of the core subunits of the SWI/SNF complex, is necessary for efficient nucleosome remodeling by BRG1 in vitro (10). SNF5 is an essential part of the esBAF (mouse embryonic stem cell specific SWI/SNF complex) and is necessary for early embryogenesis and hepatocyte differentiation (11,12). In addition, SNF5 is considered to be a tumor suppressor protein; inactivating mutations have been indentified in a large number of malignant rhabdoid tumors (13,14).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Methods for the Detection of D-Amino-Acid Oxidase
then twice for 5 min. It was incubated for 1 hr in TBS-T containing rabbit anti-hog D-amino-acid oxidase IgG (1/3,000 dilution) and quickly rinsed twice with TBS-T and further washed in TBS-T once for 15 min then twice for 5 min. The membrane was incubated
组织化学分析。（图 C）使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测；(图 D) 使用p44/42 MAPK （Erk1/2）Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注：使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。（图 E）使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit，抗体1:100 稀释；(图F)使用另一种试剂盒，抗体 1:100 稀释。
Methods for the Detection of D-Amino-Acid Oxidase
min then twice for 5 min. It was incubated for 1 hr in TBS-T containing rabbit anti-hog D-amino-acid oxidase IgG (1/3,000 dilution) and quickly rinsed twice with TBS-T and further washed in TBS-T once for 15 min then twice for 5 min. The membrane
技术资料暂无技术资料 索取技术资料
