- 询价
- Cell Signaling Technology
- 4314
- USA
- 2025年12月16日
- W, IP, IF-IC
- Rabbit
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Rab25 Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Ile185 of human Rab25 protein
- 应用范围：
W, IP, IF-IC
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 库存：
大量
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
H
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H | Endogenous | 23 | Rabbit |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Rab25 Antibody detects endogenous levels of total Rab25 protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile185 of human Rab25 protein. Western Blotting
Western blot analysis of extracts from MCF-7 and HT-29 cells using Rab25 Antibody. Western Blotting
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected (+) with human Rab25, using Rab25 Antibody. IF-IC
Confocal immunofluorescent analysis of MCF-7 cells (upper) and HeLa cells (lower), using Rab25 Antibody (green). Actin filaments were labeled using DY-554 Phallodin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Rab11a, Rab11b and Rab25 are members of the Rab11 subfamily of small Ras-like GTPases. Unlike universally expressed Rab11, typical Rab25 expression appears to be limited to gastrointestinal mucosa, kidney, and lung (1). Rab25 can associate with apical recycling vesicles to help regulate apical vesicle trafficking (2,3). Atypical Rab25 expression has been associated with various forms of cancer. Increased Rab25 expression is associated with aggressive growth in ovarian and breast cancer, where Rab25 may inhibit apoptosis and promote cancer cell proliferation and invasion through regulation of vesicle transport and cellular motility (4-7). Interaction between Rab25 and β1 integrin promotes vesicle-mediated transport of integrin to pseudopodial tip membranes, fostering the persistent invasion of tumor cells (8). Conversely, the reported loss of Rab25 expression in a number of breast cancer cases has an unclear effect on cancer pathogenesis (9).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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