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DHX29 Antibody

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  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2025年12月04日
  • W, IP
  • Rabbit
  • H,M,R
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体英文名

      DHX29 Antibody

    • 抗原

      synthetic peptide corresponding to residues at the C-terminus of human DHX29

    • 应用范围

      W, IP

    • 宿主

      Rabbit

    • 保质期

      详见说明书

    • 供应商

      CST

    • 级别

      详见MSDS文件

    • 库存

      大量

    • 适应物种

      H,M,R

    • 是否单克隆

      2

    • 保存条件

      -20°c

    • 规格

      100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting  IP=Immunoprecipitation
    Reactivity Key:  H=Human  M=Mouse  R=Rat
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Source
    W IP H M R Endogenous 155 Rabbit
    Protocols
    Specificity / Sensitivity

    DHX29 Antibody detects endogenous levels of total DHX29 protein.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the C-terminus of human DHX29. Antibodies were purified by protein A and peptide affinity chromatography.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from HeLa and HepG2 cells using DHX29 Antibody.

    Background

    DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5' untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2).

    1. de la Cruz, J. et al. (1999) Trends Biochem Sci 24, 192-8.
    2. Pisareva, V.P. et al. (2008) Cell 135, 1237-50.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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    图标文献和实验
    相关实验
    • Generation of Antibody Molecules Through Antibody Engineering

      been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional

    • The Antibody Molecule

      The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera

    • Antibody Storage

        General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease

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