Applications Key: W=Western Blotting Reactivity Key: H=Human Mk=Monkey Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Keratin 7 Antibody detects endogenous levels of keratin 7 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the amino-terminus of human keratin 7. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.
Western Blotting
Western blot analyses of extracts of various cell lines using Keratin 7 Antibody. Note that MCF7 are negative for Keratin 7, as expected.
Background
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
Keratin 7 and Keratin 19 are present in hepatic and pancreatic progenitor/stem cells (7,8).