- 询价
- Cell Signaling Technology
- 2321
- USA
- 2025年11月16日
- W, IHC-P, E-P
- All
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-(Thr) MAPK/CDK Substrate Mouse mAb
- 抗原：
synthetic phospho-threonine-proline-containing peptides
- 应用范围：
W, IHC-P, E-P
- 供应商：
CST
- 适应物种：
All
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (50 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) E-P=ELISA (Peptide)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W IHC-P E-P | All | Endogenous | Mouse IgM |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-(Thr) MAPK/CDK Substrate Mouse mAb detects phospho-threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context-independent fashion. The antibody does not cross-react with phospho-threonine in the absence of an adjacent proline. The antibody does not cross-react with phospho-tyrosine, but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e.g., phospho-Elk-1). (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with synthetic phospho-threonine-proline-containing peptides . This antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody. Antibody is purified by protein A chromatography. Western Blotting
Western blot analysis of extracts from Jurkat cells, untreated or nocodazole-treated (1 µg/ml for 12 hours prior to lysis) and subjected to 2-D gel electrophoresis, using Phospho-(Thr) MAPK/CDK Substrate Mouse mAb. Western Blotting
Western blot analysis of extracts from COS cells, untreated or treated with serum and okadaic acid, using Phospho-(Thr) MAPK/CDK Substrate Mouse mAb (left). The right panel shows total protein staining. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder, using Phospho-(Thr) MAPK/CDK Substrate Mouse mAb. IHC-P (paraffin)
Immunohistochemical analyisis of paraffin-embedded human breast carcinoma, showing staining of proteins containing phospho-threonine-proline motifs, using Phospho-(Thr) MAPK/CDK Substrate Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-(Thr) MAPK/CDK Substrate Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phospho-(Thr) MAPK/CDK Substrate Mouse mAb. ELISA-Peptide
Signal-to-noise ratio of ELISA reading using Phospho-(Thr) MAPK/CDK Substrate Mouse mAb with phospho-Thr-Pro- containing peptides (T*P) and non-Thr-Pro-containing peptides. (T* denotes phosphorylated threonine.) |
| Background | The MAPK and CDK families of serine/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To study and discover new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline. As determined by ELISA using a wide variety of phospho-Thr-Pro peptides, Phospho-(Thr) MAPK/CDK Substrate Monoclonal Antibody recognizes the phospho-Thr-Pro motif in a highly context-independent fashion. It also interacts with a broad range of phospho-Thr-Pro-containing proteins as determined by Western blot analysis of nocodazole-treated Jurkat cell extracts resolved on a 2-D gel.
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内，每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激，我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤，分别固定，打孔，猝灭，封闭，然后一抗孵育，二抗孵育，最后显色，用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正，最后得出以上数据。Quantitative
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
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