AKAP5 (D28G3) Rabbit mAb

5671:

Western Blotting

AKAP5 (D28G3) Rabbit mAb recognizes endogenous levels of total AKAP5 protein. This antibody does not cross-react with other AKAP family proteins.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg383 of human AKAP5 protein.

Western Blotting

Western blot analysis of extracts from various cell lines using AKAP5 (D28G3) Rabbit mAb.

AKAPs (A-kinase anchoring proteins), as their name implies, are a family of scaffolding proteins that bind regulatory subunits of Protein Kinase A (PKA) thus localizing PKA activity to distinct regions of the cell (1). Beyond a common amphipathic alpha helix that is responsible for recruiting the PKA regulatory subunit (RIα, RIIα, RIβ, or RIIβ), individual AKAPs contain additional domains responsible for the recrutiment of additional signaling proteins (phosphodiesterases, phosphatases, cytoskeletal components, other kinase, etc.) or restricting AKAP to a specific subcellular location (1). AKAP5 (also known as P75, AKAP75, or AKAP79) is predominantly expressed in neuronal tissues and cells where it serves to localize type II PKA to post-synaptic densities (2-4). AKAP5 specifically binds to the regulatory subunit of PKAIIβ, anchoring the enzyme to the plasma membrane and sites of cytoskeletal/membrane junctions (4-5). The other binding domains of AKAP5 have been shown to interact with calmodulin, PP2B, and calcineurin suggesting that AKAP5 may act to coordinate the cAMP- and Ca²⁺ -sensing pathways in various cell types (5-8).

1. Schwartz, J.H. (2001) Proc Natl Acad Sci U S A 98, 13482-4.
2. Bregman, D.B. et al. (1991) J Biol Chem 266, 7207-13.
3. Hirsch, A.H. et al. (1992) J Biol Chem 267, 2131-4.
4. Carr, D.W. et al. (1992) J Biol Chem 267, 16816-23.
5. Glantz, S.B. et al. (1993) J Biol Chem 268, 12796-804.
6. Klauck, T.M. et al. (1996) Science 271, 1589-92.
7. Sarkar, D. et al. (1984) J Biol Chem 259, 9840-6.
8. Coghlan, V.M. et al. (1995) Science 267, 108-11.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 5842 PKA C-α (D38C6) Rabbit mAb
• 4782 PKA C-α Antibody
• 5661 Phospho-PKA C (Thr197) (D45D3) Rabbit mAb
• 4781 Phospho-PKA C (Thr197) Antibody
• 5675 PKA RI-α (D54D9) Rabbit mAb
• 3927 PKA RI-α/β Antibody
• 9621 Phospho-(Ser/Thr) PKA Substrate Antibody
• 9624 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb
• 1984 PTMScan^® Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack

For Research Use Only. Not For Use In Diagnostic Procedures.