Toll-like Receptor 7 (D7) Rabb

5632:

Immunoprecipitation , Western Blotting

Toll-like Receptor 7 (D7) Rabbit mAb detects endogenous levels of total human TLR7 protein.

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the leucine-rich repeats within the human TLR7 protein.

Western Blotting

Western blot analysis of extracts from various cell lines using Toll-like Receptor 7 (D7) Rabbit mAb.

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human TLR7 (+), using Toll-like Receptor 7 (D7) Rabbit mAb.

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila , play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB that normally maintains NF-κB inactivity by sequestering it in the cytoplasm.

TLR7, 8 and 9 form a group of structurally related TLR family members that are are localized to intracellular endosomes (4-6). TLR7 shows highest expression in lung, placenta, and spleen (4). TLR7 mediates responses to a class of synthetic compounds, including imidazoquinolines, guanosine-based drugs that induce anti-viral responses (7). TLR7 responds to ssRNA viruses to activate NF-κB and trigger IFN production (8-10).

1. Akira, S. (2003) J Biol Chem 278, 38105-8.
2. Beutler, B. (2004) Nature 430, 257-63.
3. Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
4. Chuang, T.H. and Ulevitch, R.J. (2000) Eur Cytokine Netw 11, 372-8.
5. Du, X. et al. (2000) Eur Cytokine Netw 11, 362-71.
6. Heil, F. et al. (2003) Eur J Immunol 33, 2987-97.
7. Hemmi, H. et al. (2002) Nat Immunol 3, 196-200.
8. Heil, F. et al. (2004) Science 303, 1526-9.
9. Diebold, S.S. et al. (2004) Science 303, 1529-31.
10. Lund, J.M. et al. (2004) Proc Natl Acad Sci USA 101, 5598-603.

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• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
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For Research Use Only. Not For Use In Diagnostic Procedures.