- 询价
- Cell Signaling Technology
- 4761
- USA
- 2025年11月04日
- W
- Rabbit
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-Filamin A (Ser2152) Antibody
- 抗原：
synthetic phosphopeptides corresponding to residues surrounding Ser2152 of human filamin A and Ser2146 of human filamin C
- 应用范围：
W
- 宿主：
Rabbit
- 供应商：
CST
- 保质期：
详见说明书
- 适应物种：
H,M,R,Mk
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 280 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Filamin A (Ser2152) Antibody detects endogenous levels of filamin A only when phosphorylated at serine 2152. This antibody also reacts with filamin C when it is phosphorylated at serine 2146. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser2152 of human filamin A and Ser2146 of human filamin C. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HEK293 and HeLa cells, untreated or lamda phosphatase-treated, using Phospho-Filamin A (Ser2152) Antibody (upper) and Filamin A Antibody #4762 (lower). |
| Background | Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules (1). The actin binding domain (alpha-actinin domain) is located at the very amino-terminus, followed by as many as 24 tandem repeats of about 96 residues, and the dimerization domain is located at the carboxy-terminal end of th protein. In addition to actin filaments, filamins associate with other structural and signaling molecules such as beta-integrins, Rho/Rac/Cdc42, PKC, and insulin receptor, primarily through their carboxy-terminal half (1-3). Filamin A, the most abundant, and filamin B, less abundant, are widely expressed isoforms, while filamin C is predominantly expressed in muscle (1). Serine 2152 of filamin A is phosphorylated by PAK1, and this phosphorylation is required for PAK1-mediated actin cytoskeleton reorganization (4). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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