Troponin T (Cardiac) Antibody

5593:

Western Blotting

Troponin T (Cardiac) Antibody detects endogenous levels of total cardiac Troponin T protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Pro69 of human cardiac Troponin T protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a human cardiac Troponin T (isoform 1) construct (+), using Troponin T (Cardiac) Antibody.

Western Blotting

Western blot analysis of extracts from human and rat heart and human skeletal muscle using Troponin T (Cardiac) Antibody.

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca²⁺ concentration. Troponin consists of three subunits: the Ca²⁺ -binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca²⁺ sensitivity by reducing the Ca²⁺ binding affinity of TnC (1-3).

The tropomyosin binding subunit of the troponin complex TnT exists as different isoforms in slow skeletal muscle (ssTnT/TNNT1), fast skeletal muscle (fsTnT/TNNT3) and in cardiac muscle (cTnT/TNNT2). Each of these may also contain multiple alternatively spliced variants. Assays for measuring serum concentrations of cTnT, as well as cTnI, have been reported for analyzing cardiac injury.

1. Ward, D.G. et al. (2002) J. Biol. Chem. 277, 41795-41801.
2. Noland, T.A. et al. (1995) J. Biol. Chem. 270, 25445-25454.
3. Gaponenko, V. et al. (1999) J. Biol. Chem. 274, 16681-16684.

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• 4004 Phospho-Troponin I (Cardiac) (Ser23/24) Antibody
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• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.