Daxx (25C12) Rabbit mAb

4533:

Immunofluorescence , Western Blotting

Daxx (25C12) Rabbit mAb detects endogenous levels of total Daxx protein. While Daxx has a calculated MW of 81 kDa, it has been shown to run at an apparent MW of 110 kDa at least in part due to post-translational hyper-phosphorylation (5).

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Gln255 of Daxx.

Western Blotting

Western blot analysis of extracts from K562 (human), A20 (mouse) and PC12 (rat) cell lines using Daxx (25C12) Rabbit mAb.

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Daxx (25C12) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Daxx is a ubiquitously expressed protein that was originally identified through a yeast two-hybrid screen as an interactor with the cytoplasmic domain of Fas. It was found to enhance Fas-mediated apoptosis and activate the JNK pathway (1). However, additional studies have revealed that Daxx is actually a nuclear protein localizing to promyelocytic leukemia oncogenic domains (PODs) (2,3). Nuclear interactions have since been observed with CENP-C (4), Pax3 (5), DNA methyltransferase I (6) and chromatin-associated proteins, including histone deacetylase II, H2A, H2B, H3, H4 and Dek (7). Roles for Daxx have been suggested in transcriptional repression and cell cycle control. Loss of Daxx in mice leads to embryonic lethality with extensive developmental apoptosis, suggesting a role for Daxx directly or indirectly in suppressing cell death (5). Furthermore, inhibition of Daxx expression using RNAi has confirmed Daxx to be anti-apoptotic and to repress transcriptional activity of targets including NF-κB and E2F-1 (8).

1. Yang, X. et al. (1997) Cell 89, 1067-1076.
2. Torii, S. et al. (1999) EMBO J. 18, 6037-6049.
3. Li, H. et al. (2000) Mol. Cell Biol. 20, 1784-1796.
4. Pluta, A.F. et al. (1998) J. Cell Sci. 111, 2029-2041.
5. Hollenbach, A.D. et al. (1999) EMBO J. 18, 3702-3711.
6. Michaelson, J.A. et al. (1999) Gene Dev. 13, 1918-1923.
7. Hollenbach, A.D. et al. (2002) J. Cell Sci. 115, 3319-3330.
8. Michaelson, J.A. and Leder, P. (2003) J. Cell Sci. 116, 345-352.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.