PTMScan Phospho-Akt Substrat

Product Pathways - Motif Antibodies

• Antibodies for PTMScan^®
• more info
• application references

PTMScan^® Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads #1979

Services Information

This product is not for individual sale. It is only available as a component of the PTMScan^® Proteomics System . PTMScan^® Proteomics System orders must be priced out individually. Please email us at ptmscan@cellsignal.com to receive the most accurate pricing.

Description

PTMScan^® Immunoaffinity Beads are custom preparations of motif antibodies coupled to protein A beads. They are intended only for use for PTMScan^® and are available as components of the PTMScan^® Proteomics System.

Specificity / Sensitivity

PTMScan^® Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads detect and capture endogenous levels of peptide derived from protease digested cellular proteins containing phospho-Ser/Thr preceded by Arg at positions -5 and -3, in a manner largely independent of the surrounding amino acid sequence. Minor cross-reactivity is observed for peptides that contain phospho-Ser/Thr preceded by Arg at position -3 only. No cross-reactivity is observed with the corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Background

An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

1. Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
3. Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8.
4. Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53.
5. Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4.
6. Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

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For Research Use Only. Not For Use In Diagnostic Procedures.