- 询价
- Cell Signaling Technology
- 7469
- USA
- 2025年10月05日
- W
- Rabbit
- H,X,M,R,Mk,B,Dg
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 抗体英文名：
Cleaved Histone H3 (Thr22) Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Thr22 of human histone H3 protein
- 应用范围：
W
- 宿主：
Rabbit
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 库存：
大量
- 适应物种：
H,X,M,R,Mk,B,Dg
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey X=Xenopus B=Bovine Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H X (M) (R) (Mk) (B) (Dg) | Recombinant | 15 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Cleaved Histone H3 (Thr22) Antibody detects recombinant or enriched endogenous histone H3 protein only when cleaved in vitro with Cathepsin L at Thr22. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr22 of human histone H3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of acid-extracted histones from NTERA-2 cells (lanes 1 and 2) and recombinant Xenopus histone H3 (lanes 3 and 4), undigested (-) or digested in vitro with Cathepsin L (+), using Cleaved Histone H3 (Thr22) Antibody (upper) or Histone H3 (D1H2) XP® Rabbit mAb #4499 (lower). |
| Background | Modulation of chromatin structure has a critical role in the control of various DNA directed activities such as transcription, DNA replication, and repair (1). The basic unit of chromatin, the nucleosome, consists of two turns of DNA wrapped around two copies each of four core histone proteins (H2A, H2B, H3, and H4) (2,3). Amino-terminal tails of histones undergo various post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination in response to physiological and environmental stimuli. These modifications modulate the accessibility of chromatin to effector proteins as well as act as binding sites for specific histone modification recognizing effector proteins that regulate gene expression (1,4,5). Such alterations in chromatin modifications and architecture that accompany gene expression changes have been observed during embryonic stem cell differentiation (6). One of the ways in which chromatin modifications may be altered in stem cells involves regulated proteolysis of histone H3 by Cathepsin L. Cathepsin L cleaves the histone H3 amino-terminal tail predominantly at Thr22 in differentiating stem cells, leading to removal of histone modification marks which could then influence the expression patterns of developmentally regulated genes (7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
