- ￥2299 - 5599
- Sino Biological
- 234.375 pg/ml
- 北京
- SEK10994
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 检测范围：
234.375 pg/ml
- 检测方法：
ELISA
- 应用：
ELISA
- 适应物种：
Human
- 标记物：
HRP
- 样本：
见说明书
- 规格：
5 Plates/15 Plates
| 规格： | 5 Plates | 产品价格： | ￥2299.0 |
|---|---|---|---|
| 规格： | 15 Plates | 产品价格： | ￥5599.0 |
反应种属：Human
试剂对靶点：AGT
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：234.38-15000
试剂对标记类型：HRP
包被抗体：0.25 mg/mL of mouse anti-AGT monoclonal antibody. Dilute to a working concentration of 1.0 μg/mL in CBS before coating. (Catalog: # 10994-MM03)
检测抗体：0.5 mg/mL rabbit anti-AGT polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
试剂灵敏度：The minimum detectable dose of Human SerpinA8 / Angiotensinogen / AGT was determined to be approximately 234.375 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Human SerpinA8 / Angiotensinogen / AGT ELISA Pair Set is for the quantitative determination of Human SerpinA8 / Angiotensinogen / AGT. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for SerpinA8 / Angiotensinogen / AGT coated on a 96-well plate. Standards and samples are added to the wells, and any SerpinA8 / Angiotensinogen / AGT present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-SerpinA8 / Angiotensinogen / AGT polyclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of SerpinA8 / Angiotensinogen / AGT present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：234.375 pg/ml
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文献和实验人 Neuritin 酶联免疫分析(ELISA) 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本 中Neuritin 的含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人 Neuritin 水平。用纯化的 Neuritin 抗体 包被微孔板，制成固相抗体，往包被单抗的微孔中加入 Neuritin ，再与 HRP 标记的 Neuritin 抗体结合，形成抗体
Production and Characterization of Recombinant Human and Murine TNF
and the identity of the final products can be checked by SDS-PAGE, ELISA, Western blot, analytical gel-filtration chromatography, mass spectrometry, and lipopolysaccharide assay. The biological activity of both human and murine TNF is assessed by in vitro cytolytic
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
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