Human IL20RA / IL20R1 ELISA Pair Set(产品说明)
反应种属:Human
试剂对靶点:IL20RA
试剂对应用:ELISA
试剂对保存条件:Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围:31.25-2000
试剂对标记类型:HRP
包被抗体:0.5 mg/mL of mouse anti-IL20RA monoclonal antibody. Dilute to a working concentration of 1 μg/mL in CBS before coating. (Catalog: # 10397-MM02)
检测抗体:0.5 mg/mL rabbit anti-IL20RA polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
试剂灵敏度:The minimum detectable dose of human IL20RA / IL20R1 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理:The human IL20RA / IL20R1 ELISA Pair Set is for the quantitative determination of human IL20RA / IL20R1.This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL20RA /IL20R1 coated on a 96-well plate. Standards and samples are added to the wells,and any IL20RA / IL20R1 present binds to the immobilized antibody. The wellsare washed and a horseradish peroxidase conjugated rabbit anti-IL20RApolyclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded,which produces color in proportion to the amount of IL20RA / IL20R1 present inthe sample. To end the enzyme reaction, the stop solution is added andabsorbances of the microwell are read at 450 nm.
试剂对检测限:31.25 pg/ml