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Sino Biological Human Angiopoietin-2 ELISA Pair Set 人源ELISA检测 HRP标记

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  • ¥2299 - 5599
  • Sino Biological已认证
  • 54.69 pg/ml
  • 北京
  • SEK10691
  • 2025年08月12日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      99

    • 供应商

      北京义翘神州科技股份有限公司

    • 检测范围

      54.69 pg/ml

    • 检测方法

      ELISA

    • 应用

      ELISA

    • 适应物种

      Human

    • 标记物

      HRP

    • 样本

      见说明书

    • 规格

      5 Plates/15 Plates

    规格:5 Plates产品价格:¥2299.0
    规格:15 Plates产品价格:¥5599.0
    Human Angiopoietin-2 ELISA Pair Set(产品说明)
    反应种属:Human
    试剂对靶点:ANGPT2
    试剂对应用:ELISA
    试剂对保存条件:Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
    Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
    Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
    试剂对线性范围:54.69-3500
    试剂对标记类型:HRP
    包被抗体:1 mg/mL of mouse anti-Human Angiopoietin-2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10691-MM07)
    检测抗体:0.2 mg/mL of mouse anti-Human Angiopoietin-2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10691-MM21)
    试剂灵敏度:The minimum detectable dose of Human Angiopoietin-2 was determined to be approximately 54.69 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
    试剂对检测原理:The Human Angiopoietin-2 ELISA Pair Set is for the quantitative determination of Human Angiopoietin-2. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Angiopoietin-2 coated on a 96-well plate. Standards and samples are added to the wells, and any Human Angiopoietin-2 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human Angiopoietin-2 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Angiopoietin-2 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
    试剂对检测限:54.69 pg/ml

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