Human ECM1 ELISA Pair Set(产品说明)
反应种属:Human
试剂对靶点:ECM1
试剂对应用:ELISA
试剂对保存条件:Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围:39.06-2500
试剂对标记类型:HRP
包被抗体:1 mg/mL of mouse anti-Human ECM1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10362-MM01)
检测抗体:0.2 mg/mL of rabbit anti-Human ECM1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.8 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10362-R030)
试剂灵敏度:The minimum detectable dose of Human ECM1 was determined to be approximately 39.06 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理:The Human ECM1 ELISA Pair Set is for the quantitative determination of Human ECM1.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human ECM1 coated on a 96-well plate. Standards and samples are added to the wells, and any Human ECM1 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human ECM1 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human ECM1 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限:39.06 pg/ml
1, Reda, A; et al. Human and animal fertility studies in cystinosis reveal signs of obstructive azoospermia, an altered blood-testis barrier and a subtherapeutic effect of cysteamine in testis. Journal of inherited metabolic disease, Pubmed: 34494673
2, Drabovich, AP; et al. Differential diagnosis of azoospermia with proteomic biomarkers ECM1 and TEX101 quantified in seminal plasma. Sci Transl Med, Pubmed: 24259048