Human COMP / TSP5 ELISA Pair Set(产品说明)
反应种属:Human
试剂对靶点:COMP
试剂对应用:ELISA
试剂对保存条件:Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Streptavidin-HRP: Store at 4℃ and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
试剂对线性范围:39.06-2500
试剂对标记类型:Biotin
包被抗体:0.5 mg/mL of mouse anti-COMP monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10173-MM02)
检测抗体:Each vial contains 120 μg biotinylated rabbit anti-COMP polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 2 μg/mL in detection antibody dilution buffer before use.
试剂灵敏度:The minimum detectable dose of human COMP / TSP5 was determined to be approximately 39.1 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理:The human COMP / TSP5 ELISA Pair Set is for the quantitative determination of human COMP.This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for COMP /TSP5 coated on a 96-well plate. Standards and samples are added to the wells,and any COMP / TSP5 present binds to the immobilized antibody. The wells arewashed and a biotinylated rabbit anti-COMP polyclonal antibody is then added,producing an antibody-antigen-antibody "sandwich". To produces color inproportion to the amount of COMP / TSP5 present in the sample strepavidin-HRPand TMB substrate solution are loaded. The absorbances of the microwell areread at 450 nm.
试剂对检测限:39.1 pg/ml
1, SAPTARINI, NM; WIBOWO, MS; GUSDINAR, T. CORRELATION STUDY OF SERUM CALCIUM LEVELS AND SERUM CARTILAGE OLIGOMERIC MATRIX PROTEIN LEVELS IN RHEUMATOID ARTHRITIS PATIENTS IN BANDUNG, INDONESIA. ASIAN JOURNAL OF PHARMACEUTICAL AND CLINICAL RESEARCH