- ￥2800 - 6800
- Sino Biological
- 北京
- SEK13468
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human Ribonuclease A/RNASE1 ELISA Pair Set
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
5 Plates/15 Plates
| 规格： | 5 Plates | 产品价格： | ￥2800.0 |
|---|---|---|---|
| 规格： | 15 Plates | 产品价格： | ￥6800.0 |
Human Ribonuclease A/RNASE1 ELISA Pair Set（产品说明）
反应种属：Human
试剂对靶点：RNASE1
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：62.5-4000
试剂对标记类型：HRP
包被抗体：1 mg/mL of mouse anti-Human Ribonuclease A/RNASE1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 μg/mL in PBS before coating. (Catalog: # 13468-MM02)
检测抗体：0.2 mg/mL of rabbit anti-Human Ribonuclease A/RNASE1 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use.
试剂灵敏度：The minimum detectable dose of Human Ribonuclease A/RNASE1 was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Human Ribonuclease A/RNASE1 ELISA Pair Set is for the quantitative determination of Human Ribonuclease A/RNASE1. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Ribonuclease A/RNASE1 coated on a 96-well plate. Standards and samples are added to the wells, and any Human Ribonuclease A/RNASE1 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human Ribonuclease A/RNASE1 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Ribonuclease A/RNASE1 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：62.5 pg/ml
反应种属：Human
试剂对靶点：RNASE1
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：62.5-4000
试剂对标记类型：HRP
包被抗体：1 mg/mL of mouse anti-Human Ribonuclease A/RNASE1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 μg/mL in PBS before coating. (Catalog: # 13468-MM02)
检测抗体：0.2 mg/mL of rabbit anti-Human Ribonuclease A/RNASE1 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use.
试剂灵敏度：The minimum detectable dose of Human Ribonuclease A/RNASE1 was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Human Ribonuclease A/RNASE1 ELISA Pair Set is for the quantitative determination of Human Ribonuclease A/RNASE1. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Ribonuclease A/RNASE1 coated on a 96-well plate. Standards and samples are added to the wells, and any Human Ribonuclease A/RNASE1 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human Ribonuclease A/RNASE1 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Ribonuclease A/RNASE1 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：62.5 pg/ml
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文献和实验该产品被引用文献
1, Zechendorf E, et al. The Role of Ribonuclease 1 and Ribonuclease Inhibitor 1 in Acute Kidney Injury after Open and Endovascular Thoracoabdominal Aortic Aneurysm Repair.Journal of clinical medicine, PubMed ID: 33066382
2, Laakmann K, et al. Bacterial extracellular vesicles repress the vascular protective factor RNase1 in human lung endothelial cells.Cell communication and signaling : CCS, PubMed ID: 37189117
3, Zechendorf E, et al. A Potential Association between Ribonuclease 1 Dynamics in the Blood and the Outcome in COVID-19 Patients.International journal of molecular sciences, PubMed ID: 37569802
2, Laakmann K, et al. Bacterial extracellular vesicles repress the vascular protective factor RNase1 in human lung endothelial cells.Cell communication and signaling : CCS, PubMed ID: 37189117
3, Zechendorf E, et al. A Potential Association between Ribonuclease 1 Dynamics in the Blood and the Outcome in COVID-19 Patients.International journal of molecular sciences, PubMed ID: 37569802
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Human Ribonuclease A/RNASE1 ELISA Pair Set | 人 Ribonuclease A/RNASE1 酶联免疫配对抗体组合
￥2800 - 6800
