- ￥2800 - 6800
- Sino Biological
- 北京
- SEK11763
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human CD83 ELISA Pair Set
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
5 Plates/15 Plates
| 规格： | 5 Plates | 产品价格： | ￥2800.0 |
|---|---|---|---|
| 规格： | 15 Plates | 产品价格： | ￥6800.0 |
Human CD83 ELISA Pair Set（产品说明）
反应种属：Human
试剂对靶点：CD83
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：6.25-400
试剂对标记类型：HRP
包被抗体：0.5 mg/mL of mouse anti-Human CD83 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11763-MM11)
检测抗体：0.2 mg/mL of mouse anti-Human CD83 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11763-MM08)
试剂灵敏度：The minimum detectable dose of Human CD83 was determined to be approximately 6.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Human CD83 ELISA Pair Set is for the quantitative determination of Human CD83. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human CD83 coated on a 96-well plate. Standards and samples are added to the wells, and any Human CD83 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human CD83 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human CD83 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：6.25 pg/ml
反应种属：Human
试剂对靶点：CD83
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：6.25-400
试剂对标记类型：HRP
包被抗体：0.5 mg/mL of mouse anti-Human CD83 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11763-MM11)
检测抗体：0.2 mg/mL of mouse anti-Human CD83 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11763-MM08)
试剂灵敏度：The minimum detectable dose of Human CD83 was determined to be approximately 6.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Human CD83 ELISA Pair Set is for the quantitative determination of Human CD83. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human CD83 coated on a 96-well plate. Standards and samples are added to the wells, and any Human CD83 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human CD83 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human CD83 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：6.25 pg/ml
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文献和实验该产品被引用文献
1, Kristensen AM, et al. Expression of soluble CD83 in plasma from early-stage rheumatoid arthritis patients is not modified by anti-TNF-α therapy.Cytokine, PubMed ID: 28267648
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Human CD83 ELISA Pair Set | 人 CD83 酶联免疫配对抗体组合
￥2800 - 6800
