- ￥2800 - 6800
- Sino Biological
- 62.5 pg/ml
- 北京
- SEK40544
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 检测范围：
62.5 pg/ml
- 检测方法：
ELISA
- 应用：
ELISA
- 适应物种：
ZIKV
- 标记物：
HRP
- 样本：
见说明书
- 规格：
5 Plates/15 Plates
| 规格： | 5 Plates | 产品价格： | ￥2800.0 |
|---|---|---|---|
| 规格： | 15 Plates | 产品价格： | ￥6800.0 |
反应种属：ZIKV
试剂对靶点：ZIKV-NS1
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：62.5-4000
试剂对标记类型：HRP
包被抗体：1 mg/mL of mouse anti-Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 μg/mL in PBS before coating. (Catalog: # 40544-MM03)
检测抗体：0.2 mg/mL of mouse anti-Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 40544-MM10)
试剂灵敏度：The minimum detectable dose of Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 ELISA Pair Set is for the quantitative determination of Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 coated on a 96-well plate. Standards and samples are added to the wells, and any Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：62.5 pg/ml
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文献和实验(9), 2062-2085. 【2】LANCASTER, Madeline A., et al. Cerebral organoids model human brain development and microcephaly. Nature, 2013, 501.7467: 373-379. 【3】DANG, Jason, et al. Zika virus depletes neural progenitors in human cerebral organoids through activation
三句话读懂一篇 CNS：寒冷时食欲大增之谜；吸电子烟会增加多
Metabolism 杂志发表研究论文 Aberrant NAD+ metabolism underlies Zika virus-induced microcephaly。以多组学研究策略，揭示 ZIKV 感染导致小头症脑组织引起显著的烟酰胺腺嘌呤二核苷酸（NAD+）代谢重编程机制，以小鼠为动物模型，发现靶向干预 NAD+ 代谢路径可有效改善 ZIKV 感染所致的小头症！该研究为人类攻克寨卡病毒提供了新思路，愿该病毒早日从地球消失！图 2：来源站酷海洛3. Nature Genetics：揭示慢性肾脏病的致病
哈佛学者研发新型寨卡病毒疫苗，显著降低垂直传播风险，解决有效性欠佳问题
寨卡病毒（Zika Virus, ZIKV）自 2015 年在美洲大规模流行，其感染怀孕母体后，引发腹中胎儿小头症等发育畸形。目前已有研究报道 ZIKV 疫苗在成年未怀孕小鼠、恒河猴体内的有效性， 然而因感染 ZIKV 的母体持续产生病毒血症，迄今为止仍无安全高效的 ZIKV 疫苗完全保护母体及胎儿，研发该类疫苗的难度较大。2019 年 11 月中旬，来自哈佛医学院的 Dan H. Barouch 团队在国际期刊 Cell Host & Micorbe 上发表 ZIKV 疫苗
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