- ￥2800 - 6800
- Sino Biological
- 125 pg/ml
- 北京
- SEK40543
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 检测范围：
125 pg/ml
- 检测方法：
ELISA
- 应用：
ELISA
- 适应物种：
ZIKV
- 标记物：
HRP
- 样本：
见说明书
- 规格：
5 Plates/15 Plates
| 规格： | 5 Plates | 产品价格： | ￥2800.0 |
|---|---|---|---|
| 规格： | 15 Plates | 产品价格： | ￥6800.0 |
反应种属：ZIKV
试剂对靶点：ZIKV-E
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：125-8000
试剂对标记类型：HRP
包被抗体：0.5 mg/mL of mouse anti-Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 40543-MM35)
检测抗体：0.2 mg/mL of rabbit anti-Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.8 μg/mL in detection antibody dilution buffer before use. (Catalog: # 40543-R033)
试剂灵敏度：The minimum detectable dose of Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) was determined to be approximately 125 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) ELISA Pair Set is for the quantitative determination of Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E). The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) coated on a 96-well plate. Standards and samples are added to the wells, and any Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：125 pg/ml
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Strategies to Optimize Protein Expression in E. coli
., Anderson, D.E., Cherry, S., Copeland, T.D., and Waugh, D.S. 2001. Tobacco etch virus protease: Mechanism of autolysis and rational design of stable mutants with wild‐type catalytic proficiency. Protein Eng. 14:993‐1000
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