- ￥960
- Sino Biological
- 北京
- CV023
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
pCMV3-SP-N-His Negative Control Vector (N-terminal His-tagged)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1 Unit
Description
| Vector Name | pCMV3-SP-N-His-NCV |
| Vector Size | 6004bp |
| Vector Type | Mammalian Expression Vector |
| Expression Method | Constitutive, Stable / Transient |
| Promoter | CMV |
| Antibiotic Resistance | Kanamycin |
| Selection In Mammalian Cells | Hygromycin |
| Protein Tag | His |
| Sequencing Primer | Forward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAAGGCACAGTCGAGG) |
Schematic of pCMV3-SP-N-His-NCV (Negative Control Vector) Multiple Cloning Sites
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文献和实验, was purified in three IEC steps: a Q Sepharose Fast Flow (FF) capture step followed by a negative SP Sepharose FF step, and finally, Q Sepharose FF as a polishing step. The purification process resulted in greater than 90% pure rBoNTC(Hc )-N based on SDS
while decreasing the false-negative rate, thus increasing reliability. In this chapter, we describe a screening strategy that systematically combines N- and C-terminal fusion proteins using a recently developed vector system.
Affinity chromatography (with HIS-tagged proteins)
kit. 6)While the reactions are incubating couple your protein to the resin. We use 1mg of pure His-tagged protein per mL of resin. However, the optimal ratio may vary depending on the interaction being pursued. We treat the control resin
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