- ￥399
- Sino Biological
- 北京
- HP100730
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ 至 -80℃
- 保质期：
12个月
- 英文名：
Human SIRT2 qPCR primer pairs
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1 Unit
反应种属：Human
引物靶点：SIRT2
引物保存条件：The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.
GeneBank ID：
序列长度：0
引物描述：Verified forward and reverse primers for analyzing the quantitative expression of gene
引物质控信息：The primer mix has been verified to generate satisfactory qPCR data on Roche LightCycler480
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文献和实验PCR PRIMER DESIGN AND REACTION OPTIMISATION
Contents Factors Affecting the PCR Denaturing Temperature and Time Annealing Temperature and Primer Design Primer Length Degenerate Primers Elongation Temperature
between the primers,so the total number of fragments amplifies in an exponential fashion: 2, 4, 8,16, 32, 64, etc. Primer Design Rules primers should be at least 15 base pairs long have at least 50% G/C content anneal at a temperature in the range of 50-65 degrees
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
. The level of background QPCR signal can in addition differ between different chromatin samples, primer pairs or separate ChIP experiments. Especially if the signal obtained with particular antibodies is only moderately higher than the background, subtraction
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